Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticus for large scale Baeyer-Villiger monooxygenase reactions

Scott S. Barclay; John Woodley; Malcolm D. Lilly; Peter L. Spargo; Alan J. Pettman

doi:10.1023/A:1005610815727

Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticus for large scale Baeyer-Villiger monooxygenase reactions

Scott S. Barclay, John Woodley, Malcolm D. Lilly, Peter L. Spargo, Alan J. Pettman

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Cyclohexanone monooxygenase was produced from Acinetobacter calcoaceticus grown on a medium containing both glutamate (30 g l(-1)) and cyclohexanol (1 g l(-1)). Productivity was increased to 650 U l(-1), an order of magnitude greater than previous production methods, thereby enhancing the potential commercial utility of this enzyme.

Original language	English
Journal	Biotechnology Letters
Volume	23
Issue number	5
Pages (from-to)	385-388
ISSN	0141-5492
DOIs	https://doi.org/10.1023/A:1005610815727
Publication status	Published - 2001
Externally published	Yes

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title = "Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticus for large scale Baeyer-Villiger monooxygenase reactions",

abstract = "Cyclohexanone monooxygenase was produced from Acinetobacter calcoaceticus grown on a medium containing both glutamate (30 g l(-1)) and cyclohexanol (1 g l(-1)). Productivity was increased to 650 U l(-1), an order of magnitude greater than previous production methods, thereby enhancing the potential commercial utility of this enzyme. ",

author = "Barclay, {Scott S.} and John Woodley and Lilly, {Malcolm D.} and Spargo, {Peter L.} and Pettman, {Alan J.}",

year = "2001",

doi = "10.1023/A:1005610815727",

language = "English",

volume = "23",

pages = "385--388",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer",

number = "5",

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TY - JOUR

T1 - Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticus for large scale Baeyer-Villiger monooxygenase reactions

AU - Barclay, Scott S.

AU - Woodley, John

AU - Lilly, Malcolm D.

AU - Spargo, Peter L.

AU - Pettman, Alan J.

PY - 2001

Y1 - 2001

N2 - Cyclohexanone monooxygenase was produced from Acinetobacter calcoaceticus grown on a medium containing both glutamate (30 g l(-1)) and cyclohexanol (1 g l(-1)). Productivity was increased to 650 U l(-1), an order of magnitude greater than previous production methods, thereby enhancing the potential commercial utility of this enzyme.

AB - Cyclohexanone monooxygenase was produced from Acinetobacter calcoaceticus grown on a medium containing both glutamate (30 g l(-1)) and cyclohexanol (1 g l(-1)). Productivity was increased to 650 U l(-1), an order of magnitude greater than previous production methods, thereby enhancing the potential commercial utility of this enzyme.

U2 - 10.1023/A:1005610815727

DO - 10.1023/A:1005610815727

M3 - Journal article

VL - 23

SP - 385

EP - 388

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 5

ER -

Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticus for large scale Baeyer-Villiger monooxygenase reactions

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