Production of butyrate and caproate from a coculture of Sporomusa ovata and Clostridium kluyveri during MES

Fariza Ammam, Pier-Luc Tremblay, Neda Faraghi Parapari, Tian Zhang, Karsten Zengler

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Abstract

Microbial electrosynthesis (MES) is the process in which microorganisms use electrons derivedfrom an electrode to reduce carbon dioxide via the Wood-Ljungdahl pathway to form organiccompounds. S. ovata, a Gram-negative acetogenic bacterium is the best electrotroph organismreported to date. The lack of a genetic system for S. ovata represents an obstacle that hampersengineering of this organism for the production of organic compounds other than acetate by MES.In this study, we attempted to produce longer and valuable compounds during MES by deploying acoculture of S. ovata and C. kluyveri to bypass the lack of genetic tools. S. ovata is able to produceethanol and acetate while C. kluyveri uses these two compounds as carbon source and producesbutyrate and caproate.The first step was to optimize the growth medium for S. ovata to increase ethanol production. Theeffect of trace metal ions such as SeO4- and WO4-, as well as Ni2+, Zn2+, Cu2+, and Fe2+ on growthand ethanol production was investigated. Growth and ethanol production under autotrophicconditions (H2/CO2) were improved by increasing tungstate and nickel (WO4- and Ni2+)concentrations in the medium. The coculture S. ovata and C. kluyveri was thereafter tested underautotrophic condition (H2/CO2) using the optimised medium. A maximum of 6.0 mM and 2.0 mMof butyrate and caproate, respectively were produced after 18 days of incubation. In MES, onlytraces of butyrate were detected after 20 days. These results suggest that the amount of ethanolproduced in MES by S. ovata is not sufficient to support C. kluyveri growth; butyrate and caproateproduction. Further optimization of MES process is needed to increase ethanol production from S.ovata and subsequently increase butyrate and caproate production from C. kluyveri.
Original languageEnglish
Title of host publicationAbstract Book - DTU Sustain Conference 2014
Number of pages1
Place of PublicationKgs. Lyngby
PublisherTechnical University of Denmark
Publication date2014
Publication statusPublished - 2014
EventDTU Sustain Conference 2014 - Technical University of Denmark, Lyngby, Denmark
Duration: 17 Dec 201417 Dec 2014
http://www.sustain.dtu.dk/

Conference

ConferenceDTU Sustain Conference 2014
LocationTechnical University of Denmark
CountryDenmark
CityLyngby
Period17/12/201417/12/2014
Internet address

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