Production of a heterologous proteinase A by Saccharomyces kluyveri

Kasper Møller, L.D. Tidemand, J.R. Winther, Lisbeth Olsson, Jure Piskur, Jens Nielsen

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    In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference. S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.
    Original languageEnglish
    JournalApplied Microbiology and Biotechnology
    Issue number1-2
    Pages (from-to)216-219
    Publication statusPublished - 2001

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