Process performance and microbial community functional structure in a thermophilic anaerobic baffled reactor coupled with biocatalysed electrolysis

Tao Wang, Nan Lv, Xiaofang Pan, Chunxing Li, Gefu Zhu*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In this study, the performances of a conventional anaerobic baffled reactor (ABR) and an ABR combined with microbial electrolysis cells (MECs) for enhancing degradation of volatile fatty acids (VFAs) were evaluated in 55°C. The ABR-MECs system achieved a total chemical oxygen demand (COD) removal rate of 97.2% and a methane yield of 236 ± 5 mL g-1 CODremoved at organic loading rate (OLR) of 6.9 kg COD m-3 d-1, which were higher than those of the ABR with 77.6% and 207 ± 5 mL g-1 CODremoved, respectively, at OLR of 5.1 kg COD m-3 d-1. The pyrosequencing analysis confirmed that the introduction of MECs into ABR was conducive to establishing stable functional communities of syntrophic fatty acids oxidizing bacteria (SFOB), exoelectrogens and hydrogenotrophic methanogens, such as Syntrophobacter (5.4%), Thermodesulfovibrio (2.0%), Methanobacterium (43.8%), Methanolinea (20.4%). The content of unclassified bacteria increased from 12.4% in the ABR system to 52.3% in the ABR-MECs system. In contrast, the proportion of aceticlastic methanogens decreased from 50.1% in the ABR to 24.5% in the ABR-MECs system. The improved performance of the thermophilic ABR-MECs system resulted from phase separation, wide ecological niche and intensification of methanogenesis process via functional microbes, which significantly enhanced the degradation of propionic acid and acetic acid.
Original languageEnglish
JournalEnvironmental Technology
Number of pages11
ISSN0959-3330
DOIs
Publication statusAccepted/In press - 2020

Keywords

  • Anaerobic digestion
  • methanogenesis
  • microbial community
  • microbial electrolysis cell
  • thermophiles

Cite this

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title = "Process performance and microbial community functional structure in a thermophilic anaerobic baffled reactor coupled with biocatalysed electrolysis",
abstract = "In this study, the performances of a conventional anaerobic baffled reactor (ABR) and an ABR combined with microbial electrolysis cells (MECs) for enhancing degradation of volatile fatty acids (VFAs) were evaluated in 55°C. The ABR-MECs system achieved a total chemical oxygen demand (COD) removal rate of 97.2{\%} and a methane yield of 236 ± 5 mL g-1 CODremoved at organic loading rate (OLR) of 6.9 kg COD m-3 d-1, which were higher than those of the ABR with 77.6{\%} and 207 ± 5 mL g-1 CODremoved, respectively, at OLR of 5.1 kg COD m-3 d-1. The pyrosequencing analysis confirmed that the introduction of MECs into ABR was conducive to establishing stable functional communities of syntrophic fatty acids oxidizing bacteria (SFOB), exoelectrogens and hydrogenotrophic methanogens, such as Syntrophobacter (5.4{\%}), Thermodesulfovibrio (2.0{\%}), Methanobacterium (43.8{\%}), Methanolinea (20.4{\%}). The content of unclassified bacteria increased from 12.4{\%} in the ABR system to 52.3{\%} in the ABR-MECs system. In contrast, the proportion of aceticlastic methanogens decreased from 50.1{\%} in the ABR to 24.5{\%} in the ABR-MECs system. The improved performance of the thermophilic ABR-MECs system resulted from phase separation, wide ecological niche and intensification of methanogenesis process via functional microbes, which significantly enhanced the degradation of propionic acid and acetic acid.",
keywords = "Anaerobic digestion, methanogenesis, microbial community, microbial electrolysis cell, thermophiles",
author = "Tao Wang and Nan Lv and Xiaofang Pan and Chunxing Li and Gefu Zhu",
year = "2020",
doi = "10.1080/09593330.2018.1540664",
language = "English",
journal = "Environmental Technology",
issn = "0959-3330",
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Process performance and microbial community functional structure in a thermophilic anaerobic baffled reactor coupled with biocatalysed electrolysis. / Wang, Tao; Lv, Nan; Pan, Xiaofang; Li, Chunxing; Zhu, Gefu.

In: Environmental Technology, 2020.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Process performance and microbial community functional structure in a thermophilic anaerobic baffled reactor coupled with biocatalysed electrolysis

AU - Wang, Tao

AU - Lv, Nan

AU - Pan, Xiaofang

AU - Li, Chunxing

AU - Zhu, Gefu

PY - 2020

Y1 - 2020

N2 - In this study, the performances of a conventional anaerobic baffled reactor (ABR) and an ABR combined with microbial electrolysis cells (MECs) for enhancing degradation of volatile fatty acids (VFAs) were evaluated in 55°C. The ABR-MECs system achieved a total chemical oxygen demand (COD) removal rate of 97.2% and a methane yield of 236 ± 5 mL g-1 CODremoved at organic loading rate (OLR) of 6.9 kg COD m-3 d-1, which were higher than those of the ABR with 77.6% and 207 ± 5 mL g-1 CODremoved, respectively, at OLR of 5.1 kg COD m-3 d-1. The pyrosequencing analysis confirmed that the introduction of MECs into ABR was conducive to establishing stable functional communities of syntrophic fatty acids oxidizing bacteria (SFOB), exoelectrogens and hydrogenotrophic methanogens, such as Syntrophobacter (5.4%), Thermodesulfovibrio (2.0%), Methanobacterium (43.8%), Methanolinea (20.4%). The content of unclassified bacteria increased from 12.4% in the ABR system to 52.3% in the ABR-MECs system. In contrast, the proportion of aceticlastic methanogens decreased from 50.1% in the ABR to 24.5% in the ABR-MECs system. The improved performance of the thermophilic ABR-MECs system resulted from phase separation, wide ecological niche and intensification of methanogenesis process via functional microbes, which significantly enhanced the degradation of propionic acid and acetic acid.

AB - In this study, the performances of a conventional anaerobic baffled reactor (ABR) and an ABR combined with microbial electrolysis cells (MECs) for enhancing degradation of volatile fatty acids (VFAs) were evaluated in 55°C. The ABR-MECs system achieved a total chemical oxygen demand (COD) removal rate of 97.2% and a methane yield of 236 ± 5 mL g-1 CODremoved at organic loading rate (OLR) of 6.9 kg COD m-3 d-1, which were higher than those of the ABR with 77.6% and 207 ± 5 mL g-1 CODremoved, respectively, at OLR of 5.1 kg COD m-3 d-1. The pyrosequencing analysis confirmed that the introduction of MECs into ABR was conducive to establishing stable functional communities of syntrophic fatty acids oxidizing bacteria (SFOB), exoelectrogens and hydrogenotrophic methanogens, such as Syntrophobacter (5.4%), Thermodesulfovibrio (2.0%), Methanobacterium (43.8%), Methanolinea (20.4%). The content of unclassified bacteria increased from 12.4% in the ABR system to 52.3% in the ABR-MECs system. In contrast, the proportion of aceticlastic methanogens decreased from 50.1% in the ABR to 24.5% in the ABR-MECs system. The improved performance of the thermophilic ABR-MECs system resulted from phase separation, wide ecological niche and intensification of methanogenesis process via functional microbes, which significantly enhanced the degradation of propionic acid and acetic acid.

KW - Anaerobic digestion

KW - methanogenesis

KW - microbial community

KW - microbial electrolysis cell

KW - thermophiles

U2 - 10.1080/09593330.2018.1540664

DO - 10.1080/09593330.2018.1540664

M3 - Journal article

JO - Environmental Technology

JF - Environmental Technology

SN - 0959-3330

ER -