Probing the Role of Sigma pi Interaction and Energetics in the Catalytic Efficiency of Endo-1,4-beta-Xylanase

Raushan Kumar Singh; Manish Kumar  Tiwari; In-Won Kim; Zhilei Chen; Jung-Kul Lee

doi:10.1128/AEM.02261-12

Probing the Role of Sigma pi Interaction and Energetics in the Catalytic Efficiency of Endo-1,4-beta-Xylanase

Raushan Kumar Singh, Manish Kumar Tiwari, In-Won Kim, Zhilei Chen, Jung-Kul Lee

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Chaetomium globosum endo-1,4-beta-xylanase (XylCg) is distinguished from other xylanases by its high turnover rate (1,860 s(-1)), the highest ever reported for fungal xylanases. One conserved amino acid, W48, in the substrate binding pocket of wild-type XylCg was identified as an important residue affecting XylCg's catalytic efficiency.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	24
Pages (from-to)	8817-8821
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.02261-12
Publication status	Published - 2012
Externally published	Yes

Keywords

catalytic efficiency
energetics
enzyme activity
sigma pi interaction
turnover rate
Algae Plantae (Algae, Microorganisms, Nonvascular Plants, Plants) - Chlorophyta [13300] Chaetosphaeridium globosum species
Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Chaetomium globosum species
Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500] Aspergillus niger species Trichoderma reesei species Aspergillus versicolor species Trichoderma genus strain-SY
endo-1,4-beta-xylanase XylCg EC 3.2.1.8
03504, Genetics - Plant
10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
10802, Enzymes - General and comparative studies: coenzymes
51518, Plant physiology - Enzymes
Biochemistry and Molecular Biophysics
4386742 GenBank, EMBL, DDJB nucleotide sequence
Enzymology
Amino Acid Sequence
Amino Acid Substitution
Amino Acids
Catalytic Domain
Chaetomium
Endo-1,4-beta Xylanases
Kinetics
Models, Molecular
Molecular Sequence Data
EC 3.2.1.8 Endo-1,4-beta Xylanases
Biotechnology
Microbiology
Amino acids
amino acid
endo 1,4 beta xylanase
catalyst
chemical binding
fungus
substrate
amino acid sequence
amino acid substitution
article
chemical structure
enzyme active site
enzymology
genetics
kinetics
metabolism
molecular genetics
Chaetomium globosum
Catalytic efficiencies
Substrate binding
Turnover rate
Wild types
Xylanases
BIOTECHNOLOGY
MICROBIOLOGY
TRICHODERMA-REESEI
SUBSTRATE-BINDING
XYLANASE
SITE
FAMILY-11
SUBSITES
CHAETOMIUM globosum
Environmental Microbiology

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doi = "10.1128/AEM.02261-12",

language = "English",

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AU - Chen, Zhilei

AU - Lee, Jung-Kul

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M3 - Journal article

C2 - 23023743

SN - 0099-2240

VL - 78

SP - 8817

EP - 8821

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 24

ER -

Probing the Role of Sigma pi Interaction and Energetics in the Catalytic Efficiency of Endo-1,4-beta-Xylanase

Abstract

Keywords

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