Probing the Role of Sigma pi Interaction and Energetics in the Catalytic Efficiency of Endo-1,4-beta-Xylanase

Raushan Kumar Singh, Manish Kumar Tiwari, In-Won Kim, Zhilei Chen, Jung-Kul Lee

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Chaetomium globosum endo-1,4-beta-xylanase (XylCg) is distinguished from other xylanases by its high turnover rate (1,860 s(-1)), the highest ever reported for fungal xylanases. One conserved amino acid, W48, in the substrate binding pocket of wild-type XylCg was identified as an important residue affecting XylCg's catalytic efficiency.
Original languageEnglish
JournalAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume78
Issue number24
Pages (from-to)8817-8821
ISSN0099-2240
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • catalytic efficiency
  • energetics
  • enzyme activity
  • sigma pi interaction
  • turnover rate
  • Algae Plantae (Algae, Microorganisms, Nonvascular Plants, Plants) - Chlorophyta [13300] Chaetosphaeridium globosum species
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Chaetomium globosum species
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500] Aspergillus niger species Trichoderma reesei species Aspergillus versicolor species Trichoderma genus strain-SY
  • endo-1,4-beta-xylanase XylCg EC 3.2.1.8
  • 03504, Genetics - Plant
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 51518, Plant physiology - Enzymes
  • Biochemistry and Molecular Biophysics
  • 4386742 GenBank, EMBL, DDJB nucleotide sequence
  • Enzymology
  • Amino Acid Sequence
  • Amino Acid Substitution
  • Amino Acids
  • Catalytic Domain
  • Chaetomium
  • Endo-1,4-beta Xylanases
  • Kinetics
  • Models, Molecular
  • Molecular Sequence Data
  • EC 3.2.1.8 Endo-1,4-beta Xylanases
  • Biotechnology
  • Microbiology
  • Amino acids
  • amino acid
  • endo 1,4 beta xylanase
  • catalyst
  • chemical binding
  • fungus
  • substrate
  • amino acid sequence
  • amino acid substitution
  • article
  • chemical structure
  • enzyme active site
  • enzymology
  • genetics
  • kinetics
  • metabolism
  • molecular genetics
  • Chaetomium globosum
  • Catalytic efficiencies
  • Substrate binding
  • Turnover rate
  • Wild types
  • Xylanases
  • BIOTECHNOLOGY
  • MICROBIOLOGY
  • TRICHODERMA-REESEI
  • SUBSTRATE-BINDING
  • XYLANASE
  • SITE
  • FAMILY-11
  • SUBSITES
  • CHAETOMIUM globosum
  • Environmental Microbiology

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