Probe design for expression arrays using OligoWiz

Rasmus Wernersson

doi:10.1007/978-1-59745-538-1_2

Probe design for expression arrays using OligoWiz

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Abstract

Since all measurements from a DNA microarray is dependant on the probes used, a good choice of probes is of vital importa nce when designing custom micro-arrays. This chapter describes how to de sign expression arrays using the “
OligoWiz ” software suite. The general desired features of good probes and the issues which probe design must address are introduced and a conceptual
(rather than mathematical) description of how OligoWiz scores the quality of th
e potential probes is presented. This is followed by a detailed step-by-step guide
to designing expression arrays with OligoWiz.

Original language	English
Title of host publication	DNA Microarrays for Biomedical Research : Methods and Protocols
Editors	Martin Dufva
Volume	529
Publisher	Humana Press
Publication date	2014
Pages	23-36
Chapter	2
ISBN (Print)	978-1-934115-69-5
DOIs	https://doi.org/10.1007/978-1-59745-538-1_2
Publication status	Published - 2014

Series	Methods in Molecular Biology
Volume	529
ISSN	1064-3745

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Probe design for expression arrays using OligoWiz

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