Probe design for expression arrays using OligoWiz

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    Abstract

    Since all measurements from a DNA microarray is dependant on the probes used, a good choice of probes is of vital importa nce when designing custom micro-arrays. This chapter describes how to de sign expression arrays using the “
    OligoWiz ” software suite. The general desired features of good probes and the issues which probe design must address are introduced and a conceptual
    (rather than mathematical) description of how OligoWiz scores the quality of th
    e potential probes is presented. This is followed by a detailed step-by-step guide
    to designing expression arrays with OligoWiz.
    Original languageEnglish
    Title of host publicationDNA Microarrays for Biomedical Research : Methods and Protocols
    EditorsMartin Dufva
    Volume529
    PublisherHumana Press
    Publication date2014
    Pages23-36
    Chapter2
    ISBN (Print)978-1-934115-69-5
    DOIs
    Publication statusPublished - 2014
    SeriesMethods in Molecular Biology
    Volume529
    ISSN1064-3745

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