Principles and Applications of Flow Injection Analysis in Biosensors

Elo Harald Hansen

Research output: Contribution to journalJournal articleResearchpeer-review


In practical applications biosensors are often forced to operate under less than optimal conditions. Because of their construction, and the physical processes and chemical reactions involved in their operation, compromise conditions are frequently required to synchronize all events taking place. Therefore, and in order to implement functions such as periodic calibration, conditioning and possible regeneration of the biosensor, and, very importantly, to yield the freedom to select the optimum detection means, it is advantageous to use these devices in a flow-through mode, particularly by employing the flow injection (FI) approach. The capacity of FI, as offering itself as a complementary facility to augment the performance of biosensors, and in many cases as an attractive alternative, is demonstrated by reference to selected examples, comprising assays based on enzymatic procedures with optical and thermal detection procedures, and via description of a recently introduced technique for immunoassays, termed flow injection renewable surface immunoassays (FIRSI), which promises to entail powerful potentials and to yield compatible or better economy of operation than existing approaches.
Original languageEnglish
JournalJournal of Molecular Recognition
Issue number5/6
Pages (from-to)316-325
Publication statusPublished - 1996
Event11th International Symposium on Affinity Chromatography and Biological Recognition - San Antonio, Texas, USA
Duration: 1 Jan 1995 → …


Conference11th International Symposium on Affinity Chromatography and Biological Recognition
CitySan Antonio, Texas, USA
Period01/01/1995 → …

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