Preventive sublingual immunotherapy with House Dust Mite extract modulates epitope diversity in pre-school children

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

DOI

  • Author: Ponce, Marta

    Medical University of Vienna, Austria

  • Author: Schroeder, Fabian

    Austrian Institute of Technology, Austria

  • Author: Bannert, Christina

    Medical University of Vienna, Austria

  • Author: Schmidthaler, Klara

    Medical University of Vienna, Austria

  • Author: Hansen, Christian Skjødt

    Evaxion Biotech, Denmark

  • Author: Bøgh, Katrine Lindholm

    Research Group for Gut Microbiology and Immunology, National Food Institute, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Soldo, Regina

    Austrian Institute of Technology, Austria

  • Author: Tsui, Stephen Kwok-Wing

    Chinese University of Hong Kong, China

  • Author: Weinhäusel, Andreas

    Austrian Institute of Technology, Austria

  • Author: Szépfalusi, Zsolt

    Medical University of Vienna, Austria

  • Author: Eiwegger, Thomas

    University of Toronto, Canada

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Background The preventive effect of allergen immunotherapy (AIT) on allergy and asthma development is currently assessed using primary and secondary AIT approaches. Knowledge of the immunological effects of these interventions is limited and the impact on epitope diversity remains to be defined. Methods We used high‐density peptide arrays that included all known Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f) allergens and the whole proteome of Der f to study changes in House Dust Mite (HDM) linear peptide recognition during a 2‐year preventive double‐blind placebo‐controlled sublingual HDM AIT pilot study in 2‐5‐year‐old children with sensitization to HDM but without symptoms. Results Preventive AIT‐treated patients showed significantly higher IgG epitope diversity to HDM allergens compared to placebo‐treated individuals at 24 months of treatment (P < 0.05), while no increase in IgE diversity was seen. At 24 months of treatment, IgG4 diversity for HDM allergens was significantly higher in the pAIT‐treated patients compared to placebo group (P < 0.05). Potentially beneficial changes in epitope recognition throughout the treatment are also seen in peptides derived from Der f proteome. Conclusion These data suggest a beneficial immunomodulation of preventive sublingual immunotherapy at a molecular level by favoring a broader blocking repertoire and inhibiting epitope spreading.
Original languageEnglish
JournalAllergy
Volume74
Issue number4
Pages (from-to)780-787
ISSN0105-4538
DOIs
Publication statusPublished - 2019
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Allergen imunnity, House Dust Mite allergy, Linear epitopes, Peptide array, Preventive
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