Preventive sublingual immunotherapy with House Dust Mite extract modulates epitope diversity in pre-school children

Marta Ponce, Fabian Schroeder, Christina Bannert, Klara Schmidthaler, Christian Skjødt Hansen, Katrine Lindholm Bøgh, Regina Soldo, Stephen Kwok-Wing Tsui, Andreas Weinhäusel, Zsolt Szépfalusi, Thomas Eiwegger*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Background The preventive effect of allergen immunotherapy (AIT) on allergy and asthma development is currently assessed using primary and secondary AIT approaches. Knowledge of the immunological effects of these interventions is limited and the impact on epitope diversity remains to be defined. Methods We used high‐density peptide arrays that included all known Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f) allergens and the whole proteome of Der f to study changes in House Dust Mite (HDM) linear peptide recognition during a 2‐year preventive double‐blind placebo‐controlled sublingual HDM AIT pilot study in 2‐5‐year‐old children with sensitization to HDM but without symptoms. Results Preventive AIT‐treated patients showed significantly higher IgG epitope diversity to HDM allergens compared to placebo‐treated individuals at 24 months of treatment (P < 0.05), while no increase in IgE diversity was seen. At 24 months of treatment, IgG4 diversity for HDM allergens was significantly higher in the pAIT‐treated patients compared to placebo group (P < 0.05). Potentially beneficial changes in epitope recognition throughout the treatment are also seen in peptides derived from Der f proteome. Conclusion These data suggest a beneficial immunomodulation of preventive sublingual immunotherapy at a molecular level by favoring a broader blocking repertoire and inhibiting epitope spreading.
Original languageEnglish
JournalAllergy
Volume74
Issue number4
Pages (from-to)780-787
ISSN0105-4538
DOIs
Publication statusPublished - 2019

Keywords

  • Allergen imunnity
  • House Dust Mite allergy
  • Linear epitopes
  • Peptide array
  • Preventive

Cite this

Ponce, M., Schroeder, F., Bannert, C., Schmidthaler, K., Hansen, C. S., Bøgh, K. L., ... Eiwegger, T. (2019). Preventive sublingual immunotherapy with House Dust Mite extract modulates epitope diversity in pre-school children. Allergy, 74(4), 780-787. https://doi.org/10.1111/all.13658
Ponce, Marta ; Schroeder, Fabian ; Bannert, Christina ; Schmidthaler, Klara ; Hansen, Christian Skjødt ; Bøgh, Katrine Lindholm ; Soldo, Regina ; Tsui, Stephen Kwok-Wing ; Weinhäusel, Andreas ; Szépfalusi, Zsolt ; Eiwegger, Thomas. / Preventive sublingual immunotherapy with House Dust Mite extract modulates epitope diversity in pre-school children. In: Allergy. 2019 ; Vol. 74, No. 4. pp. 780-787.
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title = "Preventive sublingual immunotherapy with House Dust Mite extract modulates epitope diversity in pre-school children",
abstract = "Background The preventive effect of allergen immunotherapy (AIT) on allergy and asthma development is currently assessed using primary and secondary AIT approaches. Knowledge of the immunological effects of these interventions is limited and the impact on epitope diversity remains to be defined. Methods We used high‐density peptide arrays that included all known Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f) allergens and the whole proteome of Der f to study changes in House Dust Mite (HDM) linear peptide recognition during a 2‐year preventive double‐blind placebo‐controlled sublingual HDM AIT pilot study in 2‐5‐year‐old children with sensitization to HDM but without symptoms. Results Preventive AIT‐treated patients showed significantly higher IgG epitope diversity to HDM allergens compared to placebo‐treated individuals at 24 months of treatment (P < 0.05), while no increase in IgE diversity was seen. At 24 months of treatment, IgG4 diversity for HDM allergens was significantly higher in the pAIT‐treated patients compared to placebo group (P < 0.05). Potentially beneficial changes in epitope recognition throughout the treatment are also seen in peptides derived from Der f proteome. Conclusion These data suggest a beneficial immunomodulation of preventive sublingual immunotherapy at a molecular level by favoring a broader blocking repertoire and inhibiting epitope spreading.",
keywords = "Allergen imunnity, House Dust Mite allergy, Linear epitopes, Peptide array, Preventive",
author = "Marta Ponce and Fabian Schroeder and Christina Bannert and Klara Schmidthaler and Hansen, {Christian Skj{\o}dt} and B{\o}gh, {Katrine Lindholm} and Regina Soldo and Tsui, {Stephen Kwok-Wing} and Andreas Weinh{\"a}usel and Zsolt Sz{\'e}pfalusi and Thomas Eiwegger",
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pages = "780--787",
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Ponce, M, Schroeder, F, Bannert, C, Schmidthaler, K, Hansen, CS, Bøgh, KL, Soldo, R, Tsui, SK-W, Weinhäusel, A, Szépfalusi, Z & Eiwegger, T 2019, 'Preventive sublingual immunotherapy with House Dust Mite extract modulates epitope diversity in pre-school children', Allergy, vol. 74, no. 4, pp. 780-787. https://doi.org/10.1111/all.13658

Preventive sublingual immunotherapy with House Dust Mite extract modulates epitope diversity in pre-school children. / Ponce, Marta; Schroeder, Fabian; Bannert, Christina; Schmidthaler, Klara; Hansen, Christian Skjødt; Bøgh, Katrine Lindholm; Soldo, Regina; Tsui, Stephen Kwok-Wing; Weinhäusel, Andreas; Szépfalusi, Zsolt; Eiwegger, Thomas.

In: Allergy, Vol. 74, No. 4, 2019, p. 780-787.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Preventive sublingual immunotherapy with House Dust Mite extract modulates epitope diversity in pre-school children

AU - Ponce, Marta

AU - Schroeder, Fabian

AU - Bannert, Christina

AU - Schmidthaler, Klara

AU - Hansen, Christian Skjødt

AU - Bøgh, Katrine Lindholm

AU - Soldo, Regina

AU - Tsui, Stephen Kwok-Wing

AU - Weinhäusel, Andreas

AU - Szépfalusi, Zsolt

AU - Eiwegger, Thomas

PY - 2019

Y1 - 2019

N2 - Background The preventive effect of allergen immunotherapy (AIT) on allergy and asthma development is currently assessed using primary and secondary AIT approaches. Knowledge of the immunological effects of these interventions is limited and the impact on epitope diversity remains to be defined. Methods We used high‐density peptide arrays that included all known Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f) allergens and the whole proteome of Der f to study changes in House Dust Mite (HDM) linear peptide recognition during a 2‐year preventive double‐blind placebo‐controlled sublingual HDM AIT pilot study in 2‐5‐year‐old children with sensitization to HDM but without symptoms. Results Preventive AIT‐treated patients showed significantly higher IgG epitope diversity to HDM allergens compared to placebo‐treated individuals at 24 months of treatment (P < 0.05), while no increase in IgE diversity was seen. At 24 months of treatment, IgG4 diversity for HDM allergens was significantly higher in the pAIT‐treated patients compared to placebo group (P < 0.05). Potentially beneficial changes in epitope recognition throughout the treatment are also seen in peptides derived from Der f proteome. Conclusion These data suggest a beneficial immunomodulation of preventive sublingual immunotherapy at a molecular level by favoring a broader blocking repertoire and inhibiting epitope spreading.

AB - Background The preventive effect of allergen immunotherapy (AIT) on allergy and asthma development is currently assessed using primary and secondary AIT approaches. Knowledge of the immunological effects of these interventions is limited and the impact on epitope diversity remains to be defined. Methods We used high‐density peptide arrays that included all known Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f) allergens and the whole proteome of Der f to study changes in House Dust Mite (HDM) linear peptide recognition during a 2‐year preventive double‐blind placebo‐controlled sublingual HDM AIT pilot study in 2‐5‐year‐old children with sensitization to HDM but without symptoms. Results Preventive AIT‐treated patients showed significantly higher IgG epitope diversity to HDM allergens compared to placebo‐treated individuals at 24 months of treatment (P < 0.05), while no increase in IgE diversity was seen. At 24 months of treatment, IgG4 diversity for HDM allergens was significantly higher in the pAIT‐treated patients compared to placebo group (P < 0.05). Potentially beneficial changes in epitope recognition throughout the treatment are also seen in peptides derived from Der f proteome. Conclusion These data suggest a beneficial immunomodulation of preventive sublingual immunotherapy at a molecular level by favoring a broader blocking repertoire and inhibiting epitope spreading.

KW - Allergen imunnity

KW - House Dust Mite allergy

KW - Linear epitopes

KW - Peptide array

KW - Preventive

U2 - 10.1111/all.13658

DO - 10.1111/all.13658

M3 - Journal article

VL - 74

SP - 780

EP - 787

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

SN - 0105-4538

IS - 4

ER -