Prevalence of coagulase gene polymorphism in Staphylococcus aureus isolates causing bovine mastitis

Frank Møller Aarestrup, C. A. Dangler, L. M. Sordillo

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Abstract

This study was conducted to investigate polymorphism of the coagulase gene of Staphylococcus aureus causing bovine mastitis. One hundred eighty-seven strains of S. aureus were isolated from bovine mastitic milk samples obtained from 187 different Danish dairy farms. The isolates were characterised for restriction fragment length polymorphism (RFLP) of the coagulase gene. A variable region of the coagulase gene was amplified using the polymerase chain reaction (PCR) followed by AluI restriction enzyme digestion. A total of 15 different RFLP patterns were observed. The predominant pattern was found in 35% of the isolates. The ease of analysing coagulase gene polymorphisms among a large number of strains, and the multiple distinct polymorphic patterns generated, supports the use of this technique in epidemiological investigations of bovine mastitis. The predominating variants may have predelection for causing intramammary infections.
Original languageEnglish
JournalCanadian Journal of Veterinary Research
Volume59
Issue number2
Pages (from-to)124-128
ISSN0830-9000
Publication statusPublished - 1995

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