Preparation of Pooled Arabidopsis YAC DNAs for PCR-based Mapping

Henrik Næsted, Anders Bøgh Jensen, John Mundy

Research output: Contribution to journalJournal articleResearchpeer-review


Physical mapping of an Arabidopsis DNA sequence of interest can easily be performed by PCR. This is done by using specific primers and pooled DNA templates isolated from puplicly available YAC or BAC libraries. We precent simple protocols for preparing pooled YAC DNAs and PCR-Based detection of sequences in them by which several sequences can be mapped at the same time.
Original languageEnglish
JournalPlant Molecular Biology Reporter
Issue number1
Pages (from-to)61-71
Publication statusPublished - 1999
Externally publishedYes


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