Preparation of Pooled Arabidopsis YAC DNAs for PCR-based Mapping

Henrik Næsted; Anders Bøgh Jensen; John Mundy

doi:10.1023/A:1007564130408

Preparation of Pooled Arabidopsis YAC DNAs for PCR-based Mapping

Henrik Næsted, Anders Bøgh Jensen, John Mundy

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Physical mapping of an Arabidopsis DNA sequence of interest can easily be performed by PCR. This is done by using specific primers and pooled DNA templates isolated from puplicly available YAC or BAC libraries. We precent simple protocols for preparing pooled YAC DNAs and PCR-Based detection of sequences in them by which several sequences can be mapped at the same time.

Original language	English
Journal	Plant Molecular Biology Reporter
Volume	17
Issue number	1
Pages (from-to)	61-71
ISSN	0735-9640
DOIs	https://doi.org/10.1023/A:1007564130408
Publication status	Published - 1999
Externally published	Yes

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title = "Preparation of Pooled Arabidopsis YAC DNAs for PCR-based Mapping",

abstract = "Physical mapping of an Arabidopsis DNA sequence of interest can easily be performed by PCR. This is done by using specific primers and pooled DNA templates isolated from puplicly available YAC or BAC libraries. We precent simple protocols for preparing pooled YAC DNAs and PCR-Based detection of sequences in them by which several sequences can be mapped at the same time.",

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AU - Jensen, Anders Bøgh

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AB - Physical mapping of an Arabidopsis DNA sequence of interest can easily be performed by PCR. This is done by using specific primers and pooled DNA templates isolated from puplicly available YAC or BAC libraries. We precent simple protocols for preparing pooled YAC DNAs and PCR-Based detection of sequences in them by which several sequences can be mapped at the same time.

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DO - 10.1023/A:1007564130408

M3 - Journal article

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JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

SN - 0735-9640

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Preparation of Pooled Arabidopsis YAC DNAs for PCR-based Mapping

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