Abstract

Objective
In the MIMIO project (Microstructures, Microbiota, and Oral delivery), funded by The Novo Nordisk Foundation, we aim to investigate the application of a novel delivery system based on orally administered probiotic-filled microcontainers. Since loading of the microcontainers, including probiotic preparation and storage, represents a critical challenge in this project, systematic viability tests of bacteria were evaluated following various spray drying and storage conditions.
Method
The Gram-positive Lactobacillus rhamnosus GG (LGG) and Gram-negative Escherichia coli Nissle 1917 were spray dried at different settings using a BÜCHI Mini Spray Dryer, B-290. Additionally, a combination of trehalose and reconstituted skim milk (RSM) powder was added as protectant. Afterwards, the spray dried powder of LGG was stored at different conditions with variations in light exposure and temperature.
Results
For LGG, spray drying using an outlet temperature of 60°C and 10 w/w % trehalose and 10 w/w % RSM as protectants resulted in a fine powder with high cell viability, which could be stored for a prolonged period of time. However, spray drying of E. coli Nissle resulted in survival of less than 1 % at all tested conditions.
Conclusion
Spray drying with the addition of trehalose and RSM is a suitable technique to preserve LGG with a high cell viability. However, E. coli Nissle is more vulnerable to stress related to the spray drying procedure, possibly due to the sensitive Gram-negative cell wall structure. Therefore, we suggest using a different preparation method for this bacterium.
Original languageEnglish
Publication date2019
Number of pages2
Publication statusPublished - 2019
EventProbiota - Scandic Hotel, Copenhagen, Denmark
Duration: 13 Feb 201915 Feb 2019
https://www.probiotaevent.com/
https://www.probiotaevent.com/

Conference

ConferenceProbiota
LocationScandic Hotel
CountryDenmark
CityCopenhagen
Period13/02/201915/02/2019
Internet address

Cite this

@conference{0e45519278564030be6687c4daa7fa41,
title = "Preparation and storage of bacteria used for a novel probiotic delivery system",
abstract = "ObjectiveIn the MIMIO project (Microstructures, Microbiota, and Oral delivery), funded by The Novo Nordisk Foundation, we aim to investigate the application of a novel delivery system based on orally administered probiotic-filled microcontainers. Since loading of the microcontainers, including probiotic preparation and storage, represents a critical challenge in this project, systematic viability tests of bacteria were evaluated following various spray drying and storage conditions. MethodThe Gram-positive Lactobacillus rhamnosus GG (LGG) and Gram-negative Escherichia coli Nissle 1917 were spray dried at different settings using a B{\"U}CHI Mini Spray Dryer, B-290. Additionally, a combination of trehalose and reconstituted skim milk (RSM) powder was added as protectant. Afterwards, the spray dried powder of LGG was stored at different conditions with variations in light exposure and temperature. Results For LGG, spray drying using an outlet temperature of 60°C and 10 w/w {\%} trehalose and 10 w/w {\%} RSM as protectants resulted in a fine powder with high cell viability, which could be stored for a prolonged period of time. However, spray drying of E. coli Nissle resulted in survival of less than 1 {\%} at all tested conditions. ConclusionSpray drying with the addition of trehalose and RSM is a suitable technique to preserve LGG with a high cell viability. However, E. coli Nissle is more vulnerable to stress related to the spray drying procedure, possibly due to the sensitive Gram-negative cell wall structure. Therefore, we suggest using a different preparation method for this bacterium.",
author = "{Meyer Torp}, Anders and Bondegaard, {Pi Westi} and Priscila Guerra and Christfort, {Juliane Fjelrad} and Khorshid Kamguyan and Nielsen, {Line Hagner} and Anja Boisen and Licht, {Tine Rask}",
year = "2019",
language = "English",
note = "null ; Conference date: 13-02-2019 Through 15-02-2019",
url = "https://www.probiotaevent.com/, https://www.probiotaevent.com/",

}

Preparation and storage of bacteria used for a novel probiotic delivery system. / Meyer Torp, Anders; Bondegaard, Pi Westi; Guerra, Priscila; Christfort, Juliane Fjelrad; Kamguyan, Khorshid; Nielsen, Line Hagner; Boisen, Anja; Licht, Tine Rask.

2019. Abstract from Probiota, Copenhagen, Denmark.

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

TY - ABST

T1 - Preparation and storage of bacteria used for a novel probiotic delivery system

AU - Meyer Torp, Anders

AU - Bondegaard, Pi Westi

AU - Guerra, Priscila

AU - Christfort, Juliane Fjelrad

AU - Kamguyan, Khorshid

AU - Nielsen, Line Hagner

AU - Boisen, Anja

AU - Licht, Tine Rask

PY - 2019

Y1 - 2019

N2 - ObjectiveIn the MIMIO project (Microstructures, Microbiota, and Oral delivery), funded by The Novo Nordisk Foundation, we aim to investigate the application of a novel delivery system based on orally administered probiotic-filled microcontainers. Since loading of the microcontainers, including probiotic preparation and storage, represents a critical challenge in this project, systematic viability tests of bacteria were evaluated following various spray drying and storage conditions. MethodThe Gram-positive Lactobacillus rhamnosus GG (LGG) and Gram-negative Escherichia coli Nissle 1917 were spray dried at different settings using a BÜCHI Mini Spray Dryer, B-290. Additionally, a combination of trehalose and reconstituted skim milk (RSM) powder was added as protectant. Afterwards, the spray dried powder of LGG was stored at different conditions with variations in light exposure and temperature. Results For LGG, spray drying using an outlet temperature of 60°C and 10 w/w % trehalose and 10 w/w % RSM as protectants resulted in a fine powder with high cell viability, which could be stored for a prolonged period of time. However, spray drying of E. coli Nissle resulted in survival of less than 1 % at all tested conditions. ConclusionSpray drying with the addition of trehalose and RSM is a suitable technique to preserve LGG with a high cell viability. However, E. coli Nissle is more vulnerable to stress related to the spray drying procedure, possibly due to the sensitive Gram-negative cell wall structure. Therefore, we suggest using a different preparation method for this bacterium.

AB - ObjectiveIn the MIMIO project (Microstructures, Microbiota, and Oral delivery), funded by The Novo Nordisk Foundation, we aim to investigate the application of a novel delivery system based on orally administered probiotic-filled microcontainers. Since loading of the microcontainers, including probiotic preparation and storage, represents a critical challenge in this project, systematic viability tests of bacteria were evaluated following various spray drying and storage conditions. MethodThe Gram-positive Lactobacillus rhamnosus GG (LGG) and Gram-negative Escherichia coli Nissle 1917 were spray dried at different settings using a BÜCHI Mini Spray Dryer, B-290. Additionally, a combination of trehalose and reconstituted skim milk (RSM) powder was added as protectant. Afterwards, the spray dried powder of LGG was stored at different conditions with variations in light exposure and temperature. Results For LGG, spray drying using an outlet temperature of 60°C and 10 w/w % trehalose and 10 w/w % RSM as protectants resulted in a fine powder with high cell viability, which could be stored for a prolonged period of time. However, spray drying of E. coli Nissle resulted in survival of less than 1 % at all tested conditions. ConclusionSpray drying with the addition of trehalose and RSM is a suitable technique to preserve LGG with a high cell viability. However, E. coli Nissle is more vulnerable to stress related to the spray drying procedure, possibly due to the sensitive Gram-negative cell wall structure. Therefore, we suggest using a different preparation method for this bacterium.

M3 - Conference abstract for conference

ER -