Prenatal Intra-Amniotic Endotoxin Induces Fetal Gut and Lung Immune Responses and Postnatal Systemic Inflammation in Preterm Pigs

Duc Ninh Nguyen, Thomas Thymann, Sandra K. Goericke-Pesch, Shuqiang Ren, Wei Wei, Kerstin Skovgaard, Peter Damborg, Anders Brunse, Charlotte van Gorp, Boris W. Kramer, Tim G. Wolfs, Per T. Sangild*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Prenatal inflammation is a major risk for preterm birth and neonatal morbidity, but its effects on postnatal immunity and organ functions remain unclear. Using preterm pigs as a model for preterm infants, we investigated whether prenatal intra-amniotic (IA) inflammation modulates postnatal systemic immune status and organ functions. Preterm pigs exposed to IA lipopolysaccharide (LPS) for 3 days were compared with controls at birth and postnatal day 5 after formula feeding. IA LPS induced mild chorioamnionitis but extensive intra-amniotic inflammation. There were minor systemic effects at birth (increased blood neutrophil counts), but a few days later, prenatal LPS induced delayed neonatal arousal, systemic inflammation (increased blood leukocytes, plasma cytokines, and splenic bacterial counts), altered serum biochemistry (lower albumin and cholesterol and higher iron and glucose values), and increased urinary protein and sodium excretion. In the gut and lungs, IA LPS-induced inflammatory responses were observed mainly at birth (increased LPS, CXCL8, and IL-1β levels and myeloperoxidase-positive cell density, multiple increases in innate immune gene expressions, and reduced villus heights), but not on postnatal day 5 (except elevated lung CXCL8 and diarrhea symptoms). Finally, IA LPS did not affect postnatal gut brush-border enzymes, hexose absorption, permeability, or sensitivity to necrotizing enterocolitis on day 5. Short-term IA LPS exposure predisposes preterm pigs to postnatal systemic inflammation after acute fetal gut and lung inflammatory responses.
Original languageEnglish
JournalAmerican Journal of Pathology
Volume188
Issue number11
Pages (from-to)2629-2643
ISSN0002-9440
DOIs
Publication statusPublished - 2018

Cite this

Nguyen, Duc Ninh ; Thymann, Thomas ; Goericke-Pesch, Sandra K. ; Ren, Shuqiang ; Wei, Wei ; Skovgaard, Kerstin ; Damborg, Peter ; Brunse, Anders ; van Gorp, Charlotte ; Kramer, Boris W. ; Wolfs, Tim G. ; Sangild, Per T. / Prenatal Intra-Amniotic Endotoxin Induces Fetal Gut and Lung Immune Responses and Postnatal Systemic Inflammation in Preterm Pigs. In: American Journal of Pathology. 2018 ; Vol. 188, No. 11. pp. 2629-2643.
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title = "Prenatal Intra-Amniotic Endotoxin Induces Fetal Gut and Lung Immune Responses and Postnatal Systemic Inflammation in Preterm Pigs",
abstract = "Prenatal inflammation is a major risk for preterm birth and neonatal morbidity, but its effects on postnatal immunity and organ functions remain unclear. Using preterm pigs as a model for preterm infants, we investigated whether prenatal intra-amniotic (IA) inflammation modulates postnatal systemic immune status and organ functions. Preterm pigs exposed to IA lipopolysaccharide (LPS) for 3 days were compared with controls at birth and postnatal day 5 after formula feeding. IA LPS induced mild chorioamnionitis but extensive intra-amniotic inflammation. There were minor systemic effects at birth (increased blood neutrophil counts), but a few days later, prenatal LPS induced delayed neonatal arousal, systemic inflammation (increased blood leukocytes, plasma cytokines, and splenic bacterial counts), altered serum biochemistry (lower albumin and cholesterol and higher iron and glucose values), and increased urinary protein and sodium excretion. In the gut and lungs, IA LPS-induced inflammatory responses were observed mainly at birth (increased LPS, CXCL8, and IL-1β levels and myeloperoxidase-positive cell density, multiple increases in innate immune gene expressions, and reduced villus heights), but not on postnatal day 5 (except elevated lung CXCL8 and diarrhea symptoms). Finally, IA LPS did not affect postnatal gut brush-border enzymes, hexose absorption, permeability, or sensitivity to necrotizing enterocolitis on day 5. Short-term IA LPS exposure predisposes preterm pigs to postnatal systemic inflammation after acute fetal gut and lung inflammatory responses.",
author = "Nguyen, {Duc Ninh} and Thomas Thymann and Goericke-Pesch, {Sandra K.} and Shuqiang Ren and Wei Wei and Kerstin Skovgaard and Peter Damborg and Anders Brunse and {van Gorp}, Charlotte and Kramer, {Boris W.} and Wolfs, {Tim G.} and Sangild, {Per T.}",
year = "2018",
doi = "10.1016/j.ajpath.2018.07.020",
language = "English",
volume = "188",
pages = "2629--2643",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier",
number = "11",

}

Nguyen, DN, Thymann, T, Goericke-Pesch, SK, Ren, S, Wei, W, Skovgaard, K, Damborg, P, Brunse, A, van Gorp, C, Kramer, BW, Wolfs, TG & Sangild, PT 2018, 'Prenatal Intra-Amniotic Endotoxin Induces Fetal Gut and Lung Immune Responses and Postnatal Systemic Inflammation in Preterm Pigs', American Journal of Pathology, vol. 188, no. 11, pp. 2629-2643. https://doi.org/10.1016/j.ajpath.2018.07.020

Prenatal Intra-Amniotic Endotoxin Induces Fetal Gut and Lung Immune Responses and Postnatal Systemic Inflammation in Preterm Pigs. / Nguyen, Duc Ninh; Thymann, Thomas; Goericke-Pesch, Sandra K.; Ren, Shuqiang; Wei, Wei; Skovgaard, Kerstin; Damborg, Peter; Brunse, Anders; van Gorp, Charlotte; Kramer, Boris W.; Wolfs, Tim G.; Sangild, Per T.

In: American Journal of Pathology, Vol. 188, No. 11, 2018, p. 2629-2643.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Prenatal Intra-Amniotic Endotoxin Induces Fetal Gut and Lung Immune Responses and Postnatal Systemic Inflammation in Preterm Pigs

AU - Nguyen, Duc Ninh

AU - Thymann, Thomas

AU - Goericke-Pesch, Sandra K.

AU - Ren, Shuqiang

AU - Wei, Wei

AU - Skovgaard, Kerstin

AU - Damborg, Peter

AU - Brunse, Anders

AU - van Gorp, Charlotte

AU - Kramer, Boris W.

AU - Wolfs, Tim G.

AU - Sangild, Per T.

PY - 2018

Y1 - 2018

N2 - Prenatal inflammation is a major risk for preterm birth and neonatal morbidity, but its effects on postnatal immunity and organ functions remain unclear. Using preterm pigs as a model for preterm infants, we investigated whether prenatal intra-amniotic (IA) inflammation modulates postnatal systemic immune status and organ functions. Preterm pigs exposed to IA lipopolysaccharide (LPS) for 3 days were compared with controls at birth and postnatal day 5 after formula feeding. IA LPS induced mild chorioamnionitis but extensive intra-amniotic inflammation. There were minor systemic effects at birth (increased blood neutrophil counts), but a few days later, prenatal LPS induced delayed neonatal arousal, systemic inflammation (increased blood leukocytes, plasma cytokines, and splenic bacterial counts), altered serum biochemistry (lower albumin and cholesterol and higher iron and glucose values), and increased urinary protein and sodium excretion. In the gut and lungs, IA LPS-induced inflammatory responses were observed mainly at birth (increased LPS, CXCL8, and IL-1β levels and myeloperoxidase-positive cell density, multiple increases in innate immune gene expressions, and reduced villus heights), but not on postnatal day 5 (except elevated lung CXCL8 and diarrhea symptoms). Finally, IA LPS did not affect postnatal gut brush-border enzymes, hexose absorption, permeability, or sensitivity to necrotizing enterocolitis on day 5. Short-term IA LPS exposure predisposes preterm pigs to postnatal systemic inflammation after acute fetal gut and lung inflammatory responses.

AB - Prenatal inflammation is a major risk for preterm birth and neonatal morbidity, but its effects on postnatal immunity and organ functions remain unclear. Using preterm pigs as a model for preterm infants, we investigated whether prenatal intra-amniotic (IA) inflammation modulates postnatal systemic immune status and organ functions. Preterm pigs exposed to IA lipopolysaccharide (LPS) for 3 days were compared with controls at birth and postnatal day 5 after formula feeding. IA LPS induced mild chorioamnionitis but extensive intra-amniotic inflammation. There were minor systemic effects at birth (increased blood neutrophil counts), but a few days later, prenatal LPS induced delayed neonatal arousal, systemic inflammation (increased blood leukocytes, plasma cytokines, and splenic bacterial counts), altered serum biochemistry (lower albumin and cholesterol and higher iron and glucose values), and increased urinary protein and sodium excretion. In the gut and lungs, IA LPS-induced inflammatory responses were observed mainly at birth (increased LPS, CXCL8, and IL-1β levels and myeloperoxidase-positive cell density, multiple increases in innate immune gene expressions, and reduced villus heights), but not on postnatal day 5 (except elevated lung CXCL8 and diarrhea symptoms). Finally, IA LPS did not affect postnatal gut brush-border enzymes, hexose absorption, permeability, or sensitivity to necrotizing enterocolitis on day 5. Short-term IA LPS exposure predisposes preterm pigs to postnatal systemic inflammation after acute fetal gut and lung inflammatory responses.

U2 - 10.1016/j.ajpath.2018.07.020

DO - 10.1016/j.ajpath.2018.07.020

M3 - Journal article

VL - 188

SP - 2629

EP - 2643

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 11

ER -