TY - JOUR
T1 - Predictive value of cell assays for developmental toxicity and embryotoxicity of conazole fungicides.
AU - Sørensen, Karin Dreisig
AU - Taxvig, Camilla
AU - Kjærstad, Mia Birkhøj
AU - Nellemann, Christine Lydia
AU - Hass, Ulla
AU - Vinggaard, Anne Marie
PY - 2013
Y1 - 2013
N2 - This paper evaluates in vivo predictability of a battery of in vitro tests covering developmental toxicity and embryotoxicity of five widely used conazole fungicides. The conazoles were investigated in the embryonic stem cell test, and data were compared to in vivo embryotoxicity data. The same conazoles were evaluated on the basis of data from a battery of cell assays for endocrine activity, including assays for AR, ER, AhR, and sex hormone synthesis, and data were compared to in vivo developmental toxicity data. Overall, the ranking of the five conazole fungicides based on in vitro data were in reasonably good agreement with available in vivo effects. Ketoconazole and epoxiconazole are the most potent embryotoxic compounds, whereas prochloraz belongs to the most potent developmental toxicants. In conclusion, a rough prediction of the ranking of these conazole fungicides for in vivo toxicity data was possible by a holistic evaluation of data from a panel of cell-based assays.
AB - This paper evaluates in vivo predictability of a battery of in vitro tests covering developmental toxicity and embryotoxicity of five widely used conazole fungicides. The conazoles were investigated in the embryonic stem cell test, and data were compared to in vivo embryotoxicity data. The same conazoles were evaluated on the basis of data from a battery of cell assays for endocrine activity, including assays for AR, ER, AhR, and sex hormone synthesis, and data were compared to in vivo developmental toxicity data. Overall, the ranking of the five conazole fungicides based on in vitro data were in reasonably good agreement with available in vivo effects. Ketoconazole and epoxiconazole are the most potent embryotoxic compounds, whereas prochloraz belongs to the most potent developmental toxicants. In conclusion, a rough prediction of the ranking of these conazole fungicides for in vivo toxicity data was possible by a holistic evaluation of data from a panel of cell-based assays.
KW - Conazole fungicide
KW - Developmental toxicity
KW - Embryotoxicity
M3 - Journal article
SN - 1868-596X
VL - 30
SP - 319
EP - 330
JO - A L T E X. Alternatives to Animal Experimentation
JF - A L T E X. Alternatives to Animal Experimentation
IS - 3
ER -