Predicting proteasomal cleavage sites: a comparison of available methods

P. Saxova, S. Buus, Søren Brunak, Can Kesmir

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The proteasome plays an essential role in the immune responses of vertebrates. By degrading intercellular proteins from self and non-self, the proteasome produces the majority of the peptides that are presented to cytotoxic T cells (CTL). There is accumulating evidence that the C-terminal, in particular, of CTL epitopes is cleaved precisely by the proteasome, whereas the N-terminal is produced with an extension, and later trimmed by peptidases in the cytoplasm and in the endoplasmic reticulum. Recently, three publicly available methods have been developed for prediction of the specificity of the proteasome. Here, we compare the performance of these methods on a large set of CTL epitopes. The best method, NetChop at www.cbs.dtu.dk/Services/NetChop, can capture similar to70% of the C-termini correctly. This result suggests that the predictions can still be improved, particularly if more quantitative degradation data become available.
    Original languageEnglish
    JournalInternational immunology
    Volume15
    Issue number7
    Pages (from-to)781-787
    ISSN0953-8178
    Publication statusPublished - 2003

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