Predictable tuning of protein expression in bacteria

Mads Bonde; Margit Pedersen; Michael Schantz Klausen; Sheila Ingemann Jensen; Tune Wulff; Scott James Harrison; Alex Toftgaard Nielsen; Markus Herrgard; Morten Otto Alexander Sommer

doi:10.1038/nmeth.3727

Predictable tuning of protein expression in bacteria

Mads Bonde
, Margit Pedersen
, Michael Schantz Klausen
, Sheila Ingemann Jensen
, Tune Wulff
, Scott James Harrison
, Alex Toftgaard Nielsen
, Markus Herrgard
, Morten Otto Alexander Sommer

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.

Original language	English
Journal	Nature Methods
Volume	13
Pages (from-to)	233-236
Number of pages	8
ISSN	1548-7091
DOIs	https://doi.org/10.1038/nmeth.3727
Publication status	Published - 2016

Keywords

Bacteria
Gene expression
Genetic engineering

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10.1038/nmeth.3727

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title = "Predictable tuning of protein expression in bacteria",

abstract = "We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91\% of our designed sequences were within twofold of the desired target level.",

keywords = "Bacteria, Gene expression, Genetic engineering",

author = "Mads Bonde and Margit Pedersen and Klausen, \{Michael Schantz\} and \{Ingemann Jensen\}, Sheila and Tune Wulff and Harrison, \{Scott James\} and Nielsen, \{Alex Toftgaard\} and Markus Herrgard and Sommer, \{Morten Otto Alexander\}",

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doi = "10.1038/nmeth.3727",

language = "English",

volume = "13",

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journal = "Nature Methods",

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T1 - Predictable tuning of protein expression in bacteria

AU - Bonde, Mads

AU - Pedersen, Margit

AU - Klausen, Michael Schantz

AU - Ingemann Jensen, Sheila

AU - Wulff, Tune

AU - Harrison, Scott James

AU - Nielsen, Alex Toftgaard

AU - Herrgard, Markus

AU - Sommer, Morten Otto Alexander

PY - 2016

Y1 - 2016

N2 - We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.

AB - We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.

KW - Bacteria

KW - Gene expression

KW - Genetic engineering

U2 - 10.1038/nmeth.3727

DO - 10.1038/nmeth.3727

M3 - Journal article

C2 - 26752768

SN - 1548-7091

VL - 13

SP - 233

EP - 236

JO - Nature Methods

JF - Nature Methods

ER -

Predictable tuning of protein expression in bacteria

Abstract

Keywords

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