Predictable tuning of protein expression in bacteria

Research output: Contribution to journalJournal article – Annual report year: 2016Researchpeer-review

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We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.
Original languageEnglish
JournalNature Methods
Volume13
Pages (from-to)233-236
Number of pages8
ISSN1548-7091
DOIs
Publication statusPublished - 2016
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Bacteria, Gene expression, Genetic engineering

ID: 120777547