Predictable tuning of protein expression in bacteria

Mads Bonde; Margit Pedersen; Michael Schantz Klausen; Sheila Ingemann Jensen; Tune Wulff; Scott James Harrison; Alex Toftgaard Nielsen; Markus Herrgard; Morten Otto Alexander Sommer

doi:10.1038/nmeth.3727

Predictable tuning of protein expression in bacteria

Mads Bonde, Margit Pedersen, Michael Schantz Klausen, Sheila Ingemann Jensen, Tune Wulff, Scott James Harrison, Alex Toftgaard Nielsen, Markus Herrgard, Morten Otto Alexander Sommer

Novo Nordisk Foundation Center for Biosustainability

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.

Original language	English
Journal	Nature Methods
Volume	13
Pages (from-to)	233-236
Number of pages	8
ISSN	1548-7091
DOIs	https://doi.org/10.1038/nmeth.3727
Publication status	Published - 2016

Keywords

Bacteria
Gene expression
Genetic engineering

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AU - Bonde, Mads

AU - Pedersen, Margit

AU - Klausen, Michael Schantz

AU - Ingemann Jensen, Sheila

AU - Wulff, Tune

AU - Harrison, Scott James

AU - Nielsen, Alex Toftgaard

AU - Herrgard, Markus

AU - Sommer, Morten Otto Alexander

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AB - We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.

KW - Bacteria

KW - Gene expression

KW - Genetic engineering

U2 - 10.1038/nmeth.3727

DO - 10.1038/nmeth.3727

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SN - 1548-7091

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JO - Nature Methods

JF - Nature Methods

ER -

Predictable tuning of protein expression in bacteria

Abstract

Keywords

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