Predictable tuning of protein expression in bacteria

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.
Original languageEnglish
JournalNature Methods
Volume13
Pages (from-to)233-236
Number of pages8
ISSN1548-7091
DOIs
Publication statusPublished - 2016

Keywords

  • Bacteria
  • Gene expression
  • Genetic engineering

Cite this

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title = "Predictable tuning of protein expression in bacteria",
abstract = "We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91{\%} of our designed sequences were within twofold of the desired target level.",
keywords = "Bacteria, Gene expression, Genetic engineering",
author = "Mads Bonde and Margit Pedersen and Klausen, {Michael Schantz} and {Ingemann Jensen}, Sheila and Tune Wulff and Harrison, {Scott James} and Nielsen, {Alex Toftgaard} and Markus Herrgard and Sommer, {Morten Otto Alexander}",
year = "2016",
doi = "10.1038/nmeth.3727",
language = "English",
volume = "13",
pages = "233--236",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",

}

Predictable tuning of protein expression in bacteria. / Bonde, Mads; Pedersen, Margit; Klausen, Michael Schantz; Ingemann Jensen, Sheila; Wulff, Tune; Harrison, Scott James; Nielsen, Alex Toftgaard; Herrgard, Markus; Sommer, Morten Otto Alexander.

In: Nature Methods, Vol. 13, 2016, p. 233-236.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Predictable tuning of protein expression in bacteria

AU - Bonde, Mads

AU - Pedersen, Margit

AU - Klausen, Michael Schantz

AU - Ingemann Jensen, Sheila

AU - Wulff, Tune

AU - Harrison, Scott James

AU - Nielsen, Alex Toftgaard

AU - Herrgard, Markus

AU - Sommer, Morten Otto Alexander

PY - 2016

Y1 - 2016

N2 - We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.

AB - We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.

KW - Bacteria

KW - Gene expression

KW - Genetic engineering

U2 - 10.1038/nmeth.3727

DO - 10.1038/nmeth.3727

M3 - Journal article

VL - 13

SP - 233

EP - 236

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

ER -