Predictable tuning of protein expression in bacteria

Mads Bonde, Margit Pedersen, Michael Schantz Klausen, Sheila Ingemann Jensen, Tune Wulff, Scott James Harrison, Alex Toftgaard Nielsen, Markus Herrgard, Morten Otto Alexander Sommer

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.
Original languageEnglish
JournalNature Methods
Volume13
Pages (from-to)233-236
Number of pages8
ISSN1548-7091
DOIs
Publication statusPublished - 2016

Keywords

  • Bacteria
  • Gene expression
  • Genetic engineering

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