Pre-storage of gelified reagents in a lab-on-a-foil system for rapid nucleic acid analysis

Sun Yi, Jonas Høgberg, Thanner Christine, Laouenan Florian, Lisandro G. Monsalve, Sonia Rodriguez, Cuong Cao, Anders Wolff, Jesus M. Ruano-Lopez, Dang Duong Bang

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Abstract

Reagent pre-storage in a microfluidic chip can enhance operator convenience, simplify the system design, reduce the cost of storage and shipment, and avoid the risk of cross-contamination. Although dry reagents have long been used in lateral flow immunoassays, they have rarely been used for nucleic acid-based point-of-care (POC) assays due to the lack of reliable techniques to dehydrate and store fragile molecules involved in the reaction. In this study, we describe a simple and efficient method for prolonged on-chip storage of PCR reagents. The method is based on gelification of all reagents required for PCR as a ready-to-use product. The approach was successfully implemented in a lab-on-a-foil system, and the gelification process was automated for mass production. Integration of reagents on-chip by gelification greatly facilitated the development of easy-to-use lab-on-a-chip (LOC) devices for fast and cost-effective POC analysis.
Original languageEnglish
JournalLab on a Chip
Volume13
Issue number8
Pages (from-to)1509-1514
ISSN1473-0197
DOIs
Publication statusPublished - 2013

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