Potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples

Martin Weiss Nielsen , Mikael Lenz Strube, Anastasia Isbrand, Worood D. H. M. Al-Medrasi, Mette Boye, Tim Kåre Jensen, Kirstine Klitgaard Schou

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Abstract

Although treponemes are consistently identified in tissue from bovine digital dermatitis (DD) lesions, the definitive etiology of this debilitating polymicrobial disease is still unresolved. To study the microbiomes of 27 DD-infected and 10 healthy interdigital skin samples, we used a combination of different molecular methods. Deep sequencing of the 16S rRNA gene variable regions V1–V2 showed that Treponema, Mycoplasma, Fusobacterium and Porphyromonas were the genera best differentiating the DD samples from the controls. Additional deep sequencing analysis of the most abundant genus, Treponema, targeting another variable region of the 16S rRNA gene, V3–V4, identified 15 different phylotypes, among which Treponema phagedenis-like and Treponema refringens-like species were the most abundant. Although the presence of Treponema spp., Fusobacterium necrophorum and Porphyromonas levii was confirmed by fluorescence in situ hybridization (FISH), the results for Mycoplasma spp. were inconclusive. Extensive treponemal epidermal infiltration, constituting more than 90% of the total bacterial population, was observed in 24 of the 27 DD samples. F. necrophorum and P. levii were superficially located in the epidermal lesions and were present in only a subset of samples. RT-qPCR analysis showed that treponemes were also actively expressing a panel of virulence factors at the site of infection. Our results further support the hypothesis that species belonging to the genus Treponema are major pathogens of DD and also provide sufficient clues to motivate additional research into the role of M. fermentans, F. necrophorum and P. levii in the etiology of DD.
Original languageEnglish
JournalVeterinary Microbiology
Volume186
Pages (from-to)139-149
Number of pages11
ISSN0378-1135
DOIs
Publication statusPublished - 2016

Cite this

Weiss Nielsen , Martin ; Strube, Mikael Lenz ; Isbrand, Anastasia ; Al-Medrasi, Worood D. H. M. ; Boye, Mette ; Jensen, Tim Kåre ; Schou, Kirstine Klitgaard. / Potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples. In: Veterinary Microbiology. 2016 ; Vol. 186. pp. 139-149.
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title = "Potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples",
abstract = "Although treponemes are consistently identified in tissue from bovine digital dermatitis (DD) lesions, the definitive etiology of this debilitating polymicrobial disease is still unresolved. To study the microbiomes of 27 DD-infected and 10 healthy interdigital skin samples, we used a combination of different molecular methods. Deep sequencing of the 16S rRNA gene variable regions V1–V2 showed that Treponema, Mycoplasma, Fusobacterium and Porphyromonas were the genera best differentiating the DD samples from the controls. Additional deep sequencing analysis of the most abundant genus, Treponema, targeting another variable region of the 16S rRNA gene, V3–V4, identified 15 different phylotypes, among which Treponema phagedenis-like and Treponema refringens-like species were the most abundant. Although the presence of Treponema spp., Fusobacterium necrophorum and Porphyromonas levii was confirmed by fluorescence in situ hybridization (FISH), the results for Mycoplasma spp. were inconclusive. Extensive treponemal epidermal infiltration, constituting more than 90{\%} of the total bacterial population, was observed in 24 of the 27 DD samples. F. necrophorum and P. levii were superficially located in the epidermal lesions and were present in only a subset of samples. RT-qPCR analysis showed that treponemes were also actively expressing a panel of virulence factors at the site of infection. Our results further support the hypothesis that species belonging to the genus Treponema are major pathogens of DD and also provide sufficient clues to motivate additional research into the role of M. fermentans, F. necrophorum and P. levii in the etiology of DD.",
author = "{Weiss Nielsen}, Martin and Strube, {Mikael Lenz} and Anastasia Isbrand and Al-Medrasi, {Worood D. H. M.} and Mette Boye and Jensen, {Tim K{\aa}re} and Schou, {Kirstine Klitgaard}",
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Potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples. / Weiss Nielsen , Martin ; Strube, Mikael Lenz; Isbrand, Anastasia; Al-Medrasi, Worood D. H. M.; Boye, Mette; Jensen, Tim Kåre; Schou, Kirstine Klitgaard.

In: Veterinary Microbiology, Vol. 186, 2016, p. 139-149.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples

AU - Weiss Nielsen , Martin

AU - Strube, Mikael Lenz

AU - Isbrand, Anastasia

AU - Al-Medrasi, Worood D. H. M.

AU - Boye, Mette

AU - Jensen, Tim Kåre

AU - Schou, Kirstine Klitgaard

PY - 2016

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N2 - Although treponemes are consistently identified in tissue from bovine digital dermatitis (DD) lesions, the definitive etiology of this debilitating polymicrobial disease is still unresolved. To study the microbiomes of 27 DD-infected and 10 healthy interdigital skin samples, we used a combination of different molecular methods. Deep sequencing of the 16S rRNA gene variable regions V1–V2 showed that Treponema, Mycoplasma, Fusobacterium and Porphyromonas were the genera best differentiating the DD samples from the controls. Additional deep sequencing analysis of the most abundant genus, Treponema, targeting another variable region of the 16S rRNA gene, V3–V4, identified 15 different phylotypes, among which Treponema phagedenis-like and Treponema refringens-like species were the most abundant. Although the presence of Treponema spp., Fusobacterium necrophorum and Porphyromonas levii was confirmed by fluorescence in situ hybridization (FISH), the results for Mycoplasma spp. were inconclusive. Extensive treponemal epidermal infiltration, constituting more than 90% of the total bacterial population, was observed in 24 of the 27 DD samples. F. necrophorum and P. levii were superficially located in the epidermal lesions and were present in only a subset of samples. RT-qPCR analysis showed that treponemes were also actively expressing a panel of virulence factors at the site of infection. Our results further support the hypothesis that species belonging to the genus Treponema are major pathogens of DD and also provide sufficient clues to motivate additional research into the role of M. fermentans, F. necrophorum and P. levii in the etiology of DD.

AB - Although treponemes are consistently identified in tissue from bovine digital dermatitis (DD) lesions, the definitive etiology of this debilitating polymicrobial disease is still unresolved. To study the microbiomes of 27 DD-infected and 10 healthy interdigital skin samples, we used a combination of different molecular methods. Deep sequencing of the 16S rRNA gene variable regions V1–V2 showed that Treponema, Mycoplasma, Fusobacterium and Porphyromonas were the genera best differentiating the DD samples from the controls. Additional deep sequencing analysis of the most abundant genus, Treponema, targeting another variable region of the 16S rRNA gene, V3–V4, identified 15 different phylotypes, among which Treponema phagedenis-like and Treponema refringens-like species were the most abundant. Although the presence of Treponema spp., Fusobacterium necrophorum and Porphyromonas levii was confirmed by fluorescence in situ hybridization (FISH), the results for Mycoplasma spp. were inconclusive. Extensive treponemal epidermal infiltration, constituting more than 90% of the total bacterial population, was observed in 24 of the 27 DD samples. F. necrophorum and P. levii were superficially located in the epidermal lesions and were present in only a subset of samples. RT-qPCR analysis showed that treponemes were also actively expressing a panel of virulence factors at the site of infection. Our results further support the hypothesis that species belonging to the genus Treponema are major pathogens of DD and also provide sufficient clues to motivate additional research into the role of M. fermentans, F. necrophorum and P. levii in the etiology of DD.

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DO - 10.1016/j.vetmic.2016.03.003

M3 - Journal article

VL - 186

SP - 139

EP - 149

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -