Abstract
Polydimethylsiloxane (PDMS) has been used for passive equilibrium sampling in numerous abiotic environmental matrices. Recently, this approach was extended to lipid-rich tissue. This work investigated the possibilities and limitations of using PDMS thin-film extraction for in tissue equilibrium sampling in fish species of varying lipid content. Polychlorinated biphenyls (PCBs) were used as model lipophilic organic pollutants. PDMS thin-films were inserted in intact fish tissue for differing time periods (1 h up to 1 week). The thin-films were then solvent-extracted and the extracts were analyzed using gas chromatography coupled to mass spectrometry. Whether equilibrium had been established was investigated either by using PDMS thin-films of multiple thicknesses (140-620 mu m) or by assessing kinetics by means of time series. Equilibration was found to be rapid (i.e. in the range of hours) in lipid-rich fish whereas equilibrium was not achieved within one week in tissues with low or medium lipid content (i.e. up to 2% lipids). Regarding lipid-rich fish, the newly developed method was found to be sufficiently sensitive to determine equilibrium partitioning concentrations of PCBs in lipids of samples from the Baltic Sea, and it is a promising approach for any kind of fatty tissue. (C) 2009 Elsevier Ltd. All rights reserved.
Original language | English |
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Journal | Chemosphere |
Volume | 77 |
Issue number | 6 |
Pages (from-to) | 764-770 |
ISSN | 0045-6535 |
DOIs | |
Publication status | Published - 2009 |
Externally published | Yes |
Keywords
- In tissue equilibrium sampling
- Silicone thin-film extraction
- Internal exposure
- Solid Phase Microextraction (SPME)
- Persistent organic pollutants (POPs)
- Environmental monitoring