Possibilities and limitations of equilibrium sampling using polydimethylsiloxane in fish tissue

Annika Jahnke, Philipp Mayer, Dag Broman, Michael S. McLachlan

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Polydimethylsiloxane (PDMS) has been used for passive equilibrium sampling in numerous abiotic environmental matrices. Recently, this approach was extended to lipid-rich tissue. This work investigated the possibilities and limitations of using PDMS thin-film extraction for in tissue equilibrium sampling in fish species of varying lipid content. Polychlorinated biphenyls (PCBs) were used as model lipophilic organic pollutants. PDMS thin-films were inserted in intact fish tissue for differing time periods (1 h up to 1 week). The thin-films were then solvent-extracted and the extracts were analyzed using gas chromatography coupled to mass spectrometry. Whether equilibrium had been established was investigated either by using PDMS thin-films of multiple thicknesses (140-620 mu m) or by assessing kinetics by means of time series. Equilibration was found to be rapid (i.e. in the range of hours) in lipid-rich fish whereas equilibrium was not achieved within one week in tissues with low or medium lipid content (i.e. up to 2% lipids). Regarding lipid-rich fish, the newly developed method was found to be sufficiently sensitive to determine equilibrium partitioning concentrations of PCBs in lipids of samples from the Baltic Sea, and it is a promising approach for any kind of fatty tissue. (C) 2009 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalChemosphere
Volume77
Issue number6
Pages (from-to)764-770
ISSN0045-6535
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • In tissue equilibrium sampling
  • Silicone thin-film extraction
  • Internal exposure
  • Solid Phase Microextraction (SPME)
  • Persistent organic pollutants (POPs)
  • Environmental monitoring

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