Porcine reproductive and respiratory syndrome virus: Interlaboratory ring trial to evaluate real-time reverse transcription polymerase chain reaction detection methods

Research output: Contribution to journalJournal article – Annual report year: 2012Researchpeer-review

  • Author: Wernike, Kerstin

    Friedrich-Loeffler-Institute, Germany

  • Author: Bonilauri, Paolo

    Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Italy

  • Author: Dauber, Malte

    Friedrich-Loeffler-Institute, Germany

  • Author: Errington, Jane

    Animal Health and Veterinary Laboratories Agency, United Kingdom

  • Author: LeBlanc, Neil

    National Veterinary Institute, Sweden

  • Author: Revilla-Fernández, Sandra

    Österreichische Agentur für Gesundheit und Ernährungssicherheit GmbH, Austria

  • Author: Hjulsager, Charlotte Kristiane

    Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Isaksson, Mats

    National Veterinary Institute, Sweden

  • Author: Stadejek, Tomasz

    National Veterinary Research Institute, Poland

  • Author: Beer, Martin

    Friedrich-Loeffler-Institute, Germany

  • Author: Hoffmann, Bernd

    Friedrich-Loeffler-Institute, Germany

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To compare the real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays used for the diagnosis of Porcine reproductive and respiratory syndrome virus (PRRSV), a Europe-wide interlaboratory ring trial was conducted. A variety of PRRSV strains including North American (NA) and European (EU) genotype isolates were analyzed by the participants. Great differences regarding qualitative diagnostics as well as analytical sensitivity were observed between the individual RT-qPCR systems, especially when investigating strains from the EU genotype. None of the assays or commercial kits used in the ring trial could identify all different PRRSV strains with an optimal analytical and diagnostic sensitivity. The genetic variability of the PRRSV strains, which is supposed to hinder the diagnostic of the RT-PCR because of mutations at the primer binding sites, was also confirmed by sequencing and subsequent phylogenetic analysis. In summary, a major problem in PRRSV diagnostics by RT-qPCR is false-negative results. To achieve maximum safety in the molecular
diagnosis of PRRSV, the combined usage of different assays or kits is highly recommended.
Original languageEnglish
JournalJournal of Veterinary Diagnostic Investigation
Volume24
Issue number5
Pages (from-to)855–866
ISSN1040-6387
DOIs
Publication statusPublished - 2012
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Polymerase chain reaction, Porcine reproductive and respiratory syndrome virus

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