Poor Invasion of Trophoblastic Cells but Normal Plaque Formation in Fibroblastic Cells despite actA Deletion in a Group of Listeria monocytogenes Strains Persisting in Some Food Processing Environments

Anne Holch, Caroline Trebbien Gottlieb, Marianne Halberg Larsen, Hanne Ingmer, Lone Gram

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We determined mammalian cell invasion and virulence gene (inlA, inlB, and actA) sequences of Listeria monocytogenes strains belonging to a molecular subtype (RAPD 9) that often persists in Danish fish-processing plants. These strains invaded human placental trophoblasts less efficiently than other L. monocytogenes strains, including clinical strains, and they carry a premature stop codon in inlA. Eight of 15 strains, including the RAPD 9 and maternofetal strains, had a 105-nucleotide deletion in actA that did not affect cell-to-cell spread in mouse fibroblasts. The RAPD 9 strains may still be regarded as of low virulence with respect to human listeriosis.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume76
Issue number10
Pages (from-to)3391-3397
ISSN0099-2240
DOIs
Publication statusPublished - 2010

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