Polymer based biosensor for rapid electrochemical detection of virus infection of human cells

Katrine Kiilerich-Pedersen, Claus R. Poulsen, Titoo Jain, Noemi Rozlosnik

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The demand in the field of medical diagnostics for simple, cost efficient and disposable devices is growing. Here, we present a label free, all-polymer electrochemical biosensor for detection of acute viral disease. The dynamics of a viral infection in human cell culture was investigated in a micro fluidic system on conductive polymer PEDOT:TsO microelectrodes by electrochemical impedance spectroscopy and video time lapse microscopy.Employing this sensitive, real time electrochemical technique, we could measure the immediate cell response to cytomegalovirus, and detect an infection within 3h, which is several hours before the cytopathic effect is apparent with conventional imaging techniques. Atomic force microscopy and scanning ion conductance microscopy imaging consolidate the electrochemical measurements by demonstrating early virus induced changes in cell morphology of apparent programmed cell death.
    Original languageEnglish
    JournalBiosensors and Bioelectronics
    Volume28
    Issue number1
    Pages (from-to)386-392
    ISSN0956-5663
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Human cytomegalovirus (CMV)
    • Conductive polymer
    • Electrochemical impedance spectroscopy (EIS)
    • Atomic force microscopy (AFM)
    • Human fibroblast cells
    • PEDOT:TsO
    • Scanning ion conductance microscopy (SICM)

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