Poly‐L‐Lysine/Hyaluronan Nanocarriers As a Novel Nanosystem for Gene Delivery

Masoumeh Souri, Mohammad Aref Bagherzadeh, Mirza Ali Mofazzal Jahromi*, Hossein Mohammad‐Beigi, Amir Abdoli, Hamed Mir, Abazar Roustazadeh, Majid Pirestani, Parham Sahandi Zangabad, Jafar Kiani, Amirmahmoud Bakhshayesh, Mehdi Jahani, Mohammad Taghi Joghataei, Mahdi Karimi*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The present research comes up with a novel DNA-loaded poly-l-lysine (PLL) / hyaluronan (HA) nanocarrier (DNA-loaded PLL/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA-loaded PLL molecules by HA. Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission-scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM) were used to analyze the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of -24 ± 3 mV, with an average size of 138 ± 6 nm, in a ellipsoid-shape with smooth surfaces. The DNA loading efficiency (LE) measured by DNA absorbance was around 95 %. The MTT assay showed that the developed NCs are non-toxic to the cells. Furthermore,the uptake of the DNA-loaded PLL/HA NCs by the human embryonic kidney (HEK)-293T cells was evaluated by a flow cytometry method, and demonstrated high potential cellular uptake over 90% for transferring the gene to HEK-293T cells at the optimized conditions. Therefore, the DNA-loaded PLL/HA NCs are the potent strategy for developing nanosystems for gene delivery applications.
Original languageEnglish
JournalJournal of Microscopy
ISSN0022-2720
DOIs
Publication statusAccepted/In press - 2022

Keywords

  • Gene delivery
  • Nanocarriers
  • Hyaluronan
  • Poly-L-Lysine

Fingerprint

Dive into the research topics of 'Poly‐L‐Lysine/Hyaluronan Nanocarriers As a Novel Nanosystem for Gene Delivery'. Together they form a unique fingerprint.

Cite this