TY - JOUR
T1 - Poly‐L‐Lysine/Hyaluronan Nanocarriers As a Novel Nanosystem for Gene Delivery
AU - Souri, Masoumeh
AU - Bagherzadeh, Mohammad Aref
AU - Jahromi, Mirza Ali Mofazzal
AU - Mohammad‐Beigi, Hossein
AU - Abdoli, Amir
AU - Mir, Hamed
AU - Roustazadeh, Abazar
AU - Pirestani, Majid
AU - Zangabad, Parham Sahandi
AU - Kiani, Jafar
AU - Bakhshayesh, Amirmahmoud
AU - Jahani, Mehdi
AU - Joghataei, Mohammad Taghi
AU - Karimi, Mahdi
PY - 2022
Y1 - 2022
N2 - The present research comes up with a novel DNA-loaded poly-l-lysine (PLL) / hyaluronan (HA) nanocarrier (DNA-loaded PLL/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA-loaded PLL molecules by HA. Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission-scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM) were used to analyze the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of -24 ± 3 mV, with an average size of 138 ± 6 nm, in a ellipsoid-shape with smooth surfaces. The DNA loading efficiency (LE) measured by DNA absorbance was around 95 %. The MTT assay showed that the developed NCs are non-toxic to the cells. Furthermore,the uptake of the DNA-loaded PLL/HA NCs by the human embryonic kidney (HEK)-293T cells was evaluated by a flow cytometry method, and demonstrated high potential cellular uptake over 90% for transferring the gene to HEK-293T cells at the optimized conditions. Therefore, the DNA-loaded PLL/HA NCs are the potent strategy for developing nanosystems for gene delivery applications.
AB - The present research comes up with a novel DNA-loaded poly-l-lysine (PLL) / hyaluronan (HA) nanocarrier (DNA-loaded PLL/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA-loaded PLL molecules by HA. Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission-scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM) were used to analyze the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of -24 ± 3 mV, with an average size of 138 ± 6 nm, in a ellipsoid-shape with smooth surfaces. The DNA loading efficiency (LE) measured by DNA absorbance was around 95 %. The MTT assay showed that the developed NCs are non-toxic to the cells. Furthermore,the uptake of the DNA-loaded PLL/HA NCs by the human embryonic kidney (HEK)-293T cells was evaluated by a flow cytometry method, and demonstrated high potential cellular uptake over 90% for transferring the gene to HEK-293T cells at the optimized conditions. Therefore, the DNA-loaded PLL/HA NCs are the potent strategy for developing nanosystems for gene delivery applications.
KW - Gene delivery
KW - Nanocarriers
KW - Hyaluronan
KW - Poly-L-Lysine
U2 - 10.1111/jmi.13107
DO - 10.1111/jmi.13107
M3 - Journal article
C2 - 35443072
SN - 0022-2720
VL - 287
SP - 32
EP - 44
JO - Journal of Microscopy
JF - Journal of Microscopy
IS - 1
ER -