Polyketide synthase genes and molecular trade-offs in the ichthyotoxic species Prymnesium parvum

Konstantinos Anestis, Gurjeet Singh Kohli, Sylke Wohlrab, Elisabeth Varga, Thomas Ostenfeld Larsen, Per Juel Hansen, Uwe John*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Prymnesium parvum is a bloom forming haptophyte that has been responsible for numerous fish kill events across the world. The toxicity of P. parvum has been attributed to the production of large polyketide compounds, collectively called prymnesins, which based on their structure can be divided into A-, B- and C-type. The polyketide chemical nature of prymnesins indicates the potential involvement of polyketide synthases (PKSs) in their biosynthesis. However, little is known about the presence of PKSs in P. parvum as well as the potential molecular trade-offs of toxin biosynthesis. In the current study, we generated and analyzed the transcriptomes of nine P. parvum strains that produce different toxin types and have various cellular toxin contents. Numerous type I PKSs, ranging from 37 to 109, were found among the strains. Larger modular type I PKSs were mainly retrieved from strains with high cellular toxin levels and eight consensus transcripts were present in all nine strains. Gene expression variance analysis revealed potential molecular trade-offs associated with cellular toxin quantity, showing that basic metabolic processes seem to correlate negatively with cellular toxin content. These findings point towards the presence of metabolic costs for maintaining high cellular toxin quantity. The detailed analysis of PKSs in P. parvum is the first step towards better understanding the molecular basis of the biosynthesis of prymnesins and contributes to the development of molecular tools for efficient monitoring of future blooms.
Original languageEnglish
Article number18878
JournalScience of the Total Environment
Volume795
Number of pages10
ISSN0048-9697
DOIs
Publication statusPublished - 2021

Keywords

  • Prymnesis
  • Harmful algae
  • Algal toxins
  • HABs
  • Haptophyte
  • Polyketides

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