Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

Ewa Maria Musiol-Kroll, Florian Zubeil, Thomas Schafhauser, Thomas Härtner, Andreas Kulik, John McArthur, Irina Koryakina, Wolfgang Wohlleben, Stephanie Grond, Gavin J. Williams, Sang Yup Lee, Tilmann Weber

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During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by "click" chemistry.
Original languageEnglish
JournalA C S Synthetic Biology
Issue number3
Pages (from-to)421-427
Publication statusPublished - 2017


  • Antibiotic
  • Click chemistry
  • Engineering
  • Kirromycin
  • Polyketide synthase
  • Trans-acyltransferase

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Scientists discover how to make ‘click-on’ antibiotics

Tilmann Weber, Sang Yup Lee & Ewa Maria Musiol-Kroll


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Musiol-Kroll, E. M., Zubeil, F., Schafhauser, T., Härtner, T., Kulik, A., McArthur, J., Koryakina, I., Wohlleben, W., Grond, S., Williams, G. J., Lee, S. Y., & Weber, T. (2017). Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII. A C S Synthetic Biology, 6(3), 421-427. https://doi.org/10.1021/acssynbio.6b00341