Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

Ewa Maria Musiol-Kroll; Florian Zubeil; Thomas Schafhauser; Thomas Härtner; Andreas Kulik; John McArthur; Irina Koryakina; Wolfgang Wohlleben; Stephanie Grond; Gavin J.  Williams; Sang Yup Lee; Tilmann Weber

doi:10.1021/acssynbio.6b00341

Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

Ewa Maria Musiol-Kroll, Florian Zubeil, Thomas Schafhauser, Thomas Härtner, Andreas Kulik, John McArthur, Irina Koryakina, Wolfgang Wohlleben, Stephanie Grond, Gavin J. Williams, Sang Yup Lee, Tilmann Weber

Novo Nordisk Foundation Center for Biosustainability

Research output: Contribution to journal › Letter › peer-review

1 Downloads (Pure)

Abstract

During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by "click" chemistry.

Original language	English
Journal	A C S Synthetic Biology
Volume	6
Issue number	3
Pages (from-to)	421-427
ISSN	2161-5063
DOIs	https://doi.org/10.1021/acssynbio.6b00341
Publication status	Published - 2017

Keywords

Antibiotic
Click chemistry
Engineering
Kirromycin
Polyketide synthase
Trans-acyltransferase

Access to Document

10.1021/acssynbio.6b00341

http://europepmc.org/articles/pmc5483334?pdf=render

OpenUrl availability

Full text

Scientists discover how to make ‘click-on’ antibiotics
Weber, T., Lee, S. Y. & Musiol-Kroll, E. M.
04/04/2017
1 Media contribution
Press/Media: Press / Media

Cite this

@article{7cb5a54e4eb0453a8abacc5fcd3a5c69,

title = "Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII",

abstract = "During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by {"}click{"} chemistry.",

keywords = "Antibiotic, Click chemistry, Engineering, Kirromycin, Polyketide synthase, Trans-acyltransferase",

author = "Musiol-Kroll, {Ewa Maria} and Florian Zubeil and Thomas Schafhauser and Thomas H{\"a}rtner and Andreas Kulik and John McArthur and Irina Koryakina and Wolfgang Wohlleben and Stephanie Grond and Williams, {Gavin J.} and Lee, {Sang Yup} and Tilmann Weber",

year = "2017",

doi = "10.1021/acssynbio.6b00341",

language = "English",

volume = "6",

pages = "421--427",

journal = "A C S Synthetic Biology",

issn = "2161-5063",

publisher = "American Chemical Society",

number = "3",

}

TY - JOUR

T1 - Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

AU - Musiol-Kroll, Ewa Maria

AU - Zubeil, Florian

AU - Schafhauser, Thomas

AU - Härtner, Thomas

AU - Kulik, Andreas

AU - McArthur, John

AU - Koryakina, Irina

AU - Wohlleben, Wolfgang

AU - Grond, Stephanie

AU - Williams, Gavin J.

AU - Lee, Sang Yup

AU - Weber, Tilmann

PY - 2017

Y1 - 2017

N2 - During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by "click" chemistry.

AB - During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by "click" chemistry.

KW - Antibiotic

KW - Click chemistry

KW - Engineering

KW - Kirromycin

KW - Polyketide synthase

KW - Trans-acyltransferase

U2 - 10.1021/acssynbio.6b00341

DO - 10.1021/acssynbio.6b00341

M3 - Letter

C2 - 28206741

SN - 2161-5063

VL - 6

SP - 421

EP - 427

JO - A C S Synthetic Biology

JF - A C S Synthetic Biology

IS - 3

ER -

Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

Abstract

Keywords

Access to Document

OpenUrl availability

Fingerprint

Press/Media

Scientists discover how to make ‘click-on’ antibiotics

Cite this