Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

Ewa Maria Musiol-Kroll, Florian Zubeil, Thomas Schafhauser, Thomas Härtner, Andreas Kulik, John McArthur, Irina Koryakina, Wolfgang Wohlleben, Stephanie Grond, Gavin J. Williams, Sang Yup Lee, Tilmann Weber

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Abstract

During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by "click" chemistry.
Original languageEnglish
JournalA C S Synthetic Biology
Volume6
Issue number3
Pages (from-to)421-427
ISSN2161-5063
DOIs
Publication statusPublished - 2017

Keywords

  • Antibiotic
  • Click chemistry
  • Engineering
  • Kirromycin
  • Polyketide synthase
  • Trans-acyltransferase

Press / Media

Scientists discover how to make ‘click-on’ antibiotics

Tilmann Weber, Sang Yup Lee & Ewa Maria Musiol-Kroll

04/04/2017

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Cite this

Musiol-Kroll, E. M., Zubeil, F., Schafhauser, T., Härtner, T., Kulik, A., McArthur, J., Koryakina, I., Wohlleben, W., Grond, S., Williams, G. J., Lee, S. Y., & Weber, T. (2017). Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII. A C S Synthetic Biology, 6(3), 421-427. https://doi.org/10.1021/acssynbio.6b00341