Abstract
Purification of polyhydroxyalkanoates (PHA) is a challenging step, given the difficulty of achieving high PHA purity, while maintaining polymer integrity, in a sustainable and cost‐efficient manner. This study evaluated the potential of dilute ammonia digestion as a method to purify PHA from mixed microbial consortia.
RESULTSDigestion temperatures were critical to the obtainable purity and the amount of recovered PHA. At temperatures below 75 °C (regardless of the incubation time and ammonia concentration), a low PHA recovery (down to 65 %) and no increase in purity was observed. By increasing the temperature above 75 °C, a significantly higher PHA purity and higher recovery (above 90 %) could be achieved. Temperatures maximizing the purity (140 °C) led to a detrimental reduction in the molar mass of the isolated PHA, but the use of a sonication pre‐treatment enabled to increase the purity at temperatures leading to limited molar mass loss (75‐115 °C). The impurities still present in the recovered PHA did not compromise its thermal stability, and no significant degradation occurred during melting of PHA with 86 % purity (comparably to pure chloroform‐extracted PHA). Conversely, PHA recovered through H2SO4 digestion underwent severe degradation during melting, despite presenting higher purity (98 %).
CONCLUSIONS
High PHA purity, recovery and thermal stability can be obtained with dilute ammonia digestion. These observations, combined with the possibility of reusing ammonia within the process, make this method a promising approach for a more sustainable purification of PHA.
| Original language | English |
|---|---|
| Journal | Journal of Chemical Technology and Biotechnology |
| ISSN | 0268-2575 |
| Publication status | Accepted/In press - 2020 |
Cite this
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Polyhydroxyalkanoate (PHA) purification through dilute aqueous ammonia digestion at elevated temperatures. / Burniol-Figols, Anna; Skiadas, Ioannis V.; Daugaard, Anders E.; Gavala, Hariklia N.
In: Journal of Chemical Technology and Biotechnology, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Polyhydroxyalkanoate (PHA) purification through dilute aqueous ammonia digestion at elevated temperatures
AU - Burniol-Figols, Anna
AU - Skiadas, Ioannis V.
AU - Daugaard, Anders E.
AU - Gavala, Hariklia N.
PY - 2020
Y1 - 2020
N2 - BACKGROUNDPurification of polyhydroxyalkanoates (PHA) is a challenging step, given the difficulty of achieving high PHA purity, while maintaining polymer integrity, in a sustainable and cost‐efficient manner. This study evaluated the potential of dilute ammonia digestion as a method to purify PHA from mixed microbial consortia.RESULTSDigestion temperatures were critical to the obtainable purity and the amount of recovered PHA. At temperatures below 75 °C (regardless of the incubation time and ammonia concentration), a low PHA recovery (down to 65 %) and no increase in purity was observed. By increasing the temperature above 75 °C, a significantly higher PHA purity and higher recovery (above 90 %) could be achieved. Temperatures maximizing the purity (140 °C) led to a detrimental reduction in the molar mass of the isolated PHA, but the use of a sonication pre‐treatment enabled to increase the purity at temperatures leading to limited molar mass loss (75‐115 °C). The impurities still present in the recovered PHA did not compromise its thermal stability, and no significant degradation occurred during melting of PHA with 86 % purity (comparably to pure chloroform‐extracted PHA). Conversely, PHA recovered through H2SO4 digestion underwent severe degradation during melting, despite presenting higher purity (98 %).CONCLUSIONSHigh PHA purity, recovery and thermal stability can be obtained with dilute ammonia digestion. These observations, combined with the possibility of reusing ammonia within the process, make this method a promising approach for a more sustainable purification of PHA.
AB - BACKGROUNDPurification of polyhydroxyalkanoates (PHA) is a challenging step, given the difficulty of achieving high PHA purity, while maintaining polymer integrity, in a sustainable and cost‐efficient manner. This study evaluated the potential of dilute ammonia digestion as a method to purify PHA from mixed microbial consortia.RESULTSDigestion temperatures were critical to the obtainable purity and the amount of recovered PHA. At temperatures below 75 °C (regardless of the incubation time and ammonia concentration), a low PHA recovery (down to 65 %) and no increase in purity was observed. By increasing the temperature above 75 °C, a significantly higher PHA purity and higher recovery (above 90 %) could be achieved. Temperatures maximizing the purity (140 °C) led to a detrimental reduction in the molar mass of the isolated PHA, but the use of a sonication pre‐treatment enabled to increase the purity at temperatures leading to limited molar mass loss (75‐115 °C). The impurities still present in the recovered PHA did not compromise its thermal stability, and no significant degradation occurred during melting of PHA with 86 % purity (comparably to pure chloroform‐extracted PHA). Conversely, PHA recovered through H2SO4 digestion underwent severe degradation during melting, despite presenting higher purity (98 %).CONCLUSIONSHigh PHA purity, recovery and thermal stability can be obtained with dilute ammonia digestion. These observations, combined with the possibility of reusing ammonia within the process, make this method a promising approach for a more sustainable purification of PHA.
M3 - Journal article
JO - Journal of Chemical Technology and Biotechnology
JF - Journal of Chemical Technology and Biotechnology
SN - 0268-2575
ER -