Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal

Juliette Guitard, Pernille Andersen, Caroline Ermont, Sedami Gnidehou, Nadine Fievet, Ole Lund, Philippe Deloron, Nicaise Tuikue Ndam

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    Abstract

    Background: Pregnant women acquire protective antibodies that cross-react with geographically diverse placental Plasmodium falciparum isolates, suggesting that surface molecules expressed on infected erythrocytes by pregnancy-associated malaria (PAM) parasites have conserved epitopes and, that designing a PAM vaccine may be envisaged. VAR2CSA is the main candidate for a pregnancy malaria vaccine, but vaccine development may be complicated by its sequence polymorphism. Methods: The dynamics of P. falciparum genotypes during pregnancy in 32 women in relation to VAR2CSA polymorphism and immunity was determined. The polymorphism of the msp2 gene and five microsatellites was analysed in consecutive parasite isolates, and the DBL5 epsilon + Interdomain 5 (Id5) part of the var2csa gene of the corresponding samples was cloned and sequenced to measure variation. Results: In primigravidae, the multiplicity of infection in the placenta was associated with occurrence of low birth weight babies. Some parasite genotypes were able to persist over several weeks and, still be present in the placenta at delivery particularly when the host anti-VAR2CSA antibody level was low. Comparison of diversity among genotyping markers confirmed that some PAM parasites may harbour more than one var2csa gene copy in their genome. Conclusions: Host immunity to VAR2CSA influences the parasite dynamics during pregnancy, suggesting that the acquisition of protective immunity requires pre-exposure to a limited number of parasite variants. Presence of highly conserved residues in surface-exposed areas of the VAR2CSA immunodominant DBL5 epsilon domain, suggest its potential in inducing antibodies with broad reactivity.
    Original languageEnglish
    JournalMalaria Journal
    Volume9
    Issue number165
    ISSN1475-2875
    DOIs
    Publication statusPublished - 2010

    Bibliographical note

    This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Cite this

    Guitard, J., Andersen, P., Ermont, C., Gnidehou, S., Fievet, N., Lund, O., ... Ndam, N. T. (2010). Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal. Malaria Journal, 9(165). https://doi.org/10.1186/1475-2875-9-165
    Guitard, Juliette ; Andersen, Pernille ; Ermont, Caroline ; Gnidehou, Sedami ; Fievet, Nadine ; Lund, Ole ; Deloron, Philippe ; Ndam, Nicaise Tuikue. / Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal. In: Malaria Journal. 2010 ; Vol. 9, No. 165.
    @article{56e6febc9162419f9fec25c23c31e77b,
    title = "Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal",
    abstract = "Background: Pregnant women acquire protective antibodies that cross-react with geographically diverse placental Plasmodium falciparum isolates, suggesting that surface molecules expressed on infected erythrocytes by pregnancy-associated malaria (PAM) parasites have conserved epitopes and, that designing a PAM vaccine may be envisaged. VAR2CSA is the main candidate for a pregnancy malaria vaccine, but vaccine development may be complicated by its sequence polymorphism. Methods: The dynamics of P. falciparum genotypes during pregnancy in 32 women in relation to VAR2CSA polymorphism and immunity was determined. The polymorphism of the msp2 gene and five microsatellites was analysed in consecutive parasite isolates, and the DBL5 epsilon + Interdomain 5 (Id5) part of the var2csa gene of the corresponding samples was cloned and sequenced to measure variation. Results: In primigravidae, the multiplicity of infection in the placenta was associated with occurrence of low birth weight babies. Some parasite genotypes were able to persist over several weeks and, still be present in the placenta at delivery particularly when the host anti-VAR2CSA antibody level was low. Comparison of diversity among genotyping markers confirmed that some PAM parasites may harbour more than one var2csa gene copy in their genome. Conclusions: Host immunity to VAR2CSA influences the parasite dynamics during pregnancy, suggesting that the acquisition of protective immunity requires pre-exposure to a limited number of parasite variants. Presence of highly conserved residues in surface-exposed areas of the VAR2CSA immunodominant DBL5 epsilon domain, suggest its potential in inducing antibodies with broad reactivity.",
    author = "Juliette Guitard and Pernille Andersen and Caroline Ermont and Sedami Gnidehou and Nadine Fievet and Ole Lund and Philippe Deloron and Ndam, {Nicaise Tuikue}",
    note = "This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.",
    year = "2010",
    doi = "10.1186/1475-2875-9-165",
    language = "English",
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    journal = "Malaria Journal",
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    Guitard, J, Andersen, P, Ermont, C, Gnidehou, S, Fievet, N, Lund, O, Deloron, P & Ndam, NT 2010, 'Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal', Malaria Journal, vol. 9, no. 165. https://doi.org/10.1186/1475-2875-9-165

    Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal. / Guitard, Juliette; Andersen, Pernille; Ermont, Caroline; Gnidehou, Sedami; Fievet, Nadine; Lund, Ole; Deloron, Philippe; Ndam, Nicaise Tuikue.

    In: Malaria Journal, Vol. 9, No. 165, 2010.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal

    AU - Guitard, Juliette

    AU - Andersen, Pernille

    AU - Ermont, Caroline

    AU - Gnidehou, Sedami

    AU - Fievet, Nadine

    AU - Lund, Ole

    AU - Deloron, Philippe

    AU - Ndam, Nicaise Tuikue

    N1 - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

    PY - 2010

    Y1 - 2010

    N2 - Background: Pregnant women acquire protective antibodies that cross-react with geographically diverse placental Plasmodium falciparum isolates, suggesting that surface molecules expressed on infected erythrocytes by pregnancy-associated malaria (PAM) parasites have conserved epitopes and, that designing a PAM vaccine may be envisaged. VAR2CSA is the main candidate for a pregnancy malaria vaccine, but vaccine development may be complicated by its sequence polymorphism. Methods: The dynamics of P. falciparum genotypes during pregnancy in 32 women in relation to VAR2CSA polymorphism and immunity was determined. The polymorphism of the msp2 gene and five microsatellites was analysed in consecutive parasite isolates, and the DBL5 epsilon + Interdomain 5 (Id5) part of the var2csa gene of the corresponding samples was cloned and sequenced to measure variation. Results: In primigravidae, the multiplicity of infection in the placenta was associated with occurrence of low birth weight babies. Some parasite genotypes were able to persist over several weeks and, still be present in the placenta at delivery particularly when the host anti-VAR2CSA antibody level was low. Comparison of diversity among genotyping markers confirmed that some PAM parasites may harbour more than one var2csa gene copy in their genome. Conclusions: Host immunity to VAR2CSA influences the parasite dynamics during pregnancy, suggesting that the acquisition of protective immunity requires pre-exposure to a limited number of parasite variants. Presence of highly conserved residues in surface-exposed areas of the VAR2CSA immunodominant DBL5 epsilon domain, suggest its potential in inducing antibodies with broad reactivity.

    AB - Background: Pregnant women acquire protective antibodies that cross-react with geographically diverse placental Plasmodium falciparum isolates, suggesting that surface molecules expressed on infected erythrocytes by pregnancy-associated malaria (PAM) parasites have conserved epitopes and, that designing a PAM vaccine may be envisaged. VAR2CSA is the main candidate for a pregnancy malaria vaccine, but vaccine development may be complicated by its sequence polymorphism. Methods: The dynamics of P. falciparum genotypes during pregnancy in 32 women in relation to VAR2CSA polymorphism and immunity was determined. The polymorphism of the msp2 gene and five microsatellites was analysed in consecutive parasite isolates, and the DBL5 epsilon + Interdomain 5 (Id5) part of the var2csa gene of the corresponding samples was cloned and sequenced to measure variation. Results: In primigravidae, the multiplicity of infection in the placenta was associated with occurrence of low birth weight babies. Some parasite genotypes were able to persist over several weeks and, still be present in the placenta at delivery particularly when the host anti-VAR2CSA antibody level was low. Comparison of diversity among genotyping markers confirmed that some PAM parasites may harbour more than one var2csa gene copy in their genome. Conclusions: Host immunity to VAR2CSA influences the parasite dynamics during pregnancy, suggesting that the acquisition of protective immunity requires pre-exposure to a limited number of parasite variants. Presence of highly conserved residues in surface-exposed areas of the VAR2CSA immunodominant DBL5 epsilon domain, suggest its potential in inducing antibodies with broad reactivity.

    U2 - 10.1186/1475-2875-9-165

    DO - 10.1186/1475-2875-9-165

    M3 - Journal article

    C2 - 20553578

    VL - 9

    JO - Malaria Journal

    JF - Malaria Journal

    SN - 1475-2875

    IS - 165

    ER -