Plasmid profiles, restriction fragment length polymorphisms and O-serotypes among Vibrio anguillarum isolates

Karl Pedersen, T. Tiainen, J.L. Larsen

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Abstract

A total of 279 Vibrio anguillarum strains were serotyped and examined for plasmid content. Plasmids were subjected to digestion with restriction enzymes. Most strains belonged to serogroup O1 (39%) and O2 (16%). In total 164 strains (53%) carried plasmids. Of the O1 and O2 isolates, 92% and 30%, respectively, carried one or more plasmids. Restriction fragment length polymorphism (RFLP) analysis of plasmid DNA indicated that plasmids belonged to several groups. Each group seemed to be restricted to a single O-serovar. The largest group was the pJM1-like plasmids among most serovar O1 strains. Most of these plasmids were about 67 kb like the pJM1 plasmid, but various derivatives ranged from 26-77 kb. RFLP studies of the 67 kb plasmids revealed 17 different restriction patterns. Some patterns were dominant among European strains whereas others were dominant among North American strains. The results confirmed the applicability of O-serotyping together with plasmid profile and restriction analysis of plasmids for typing of V. anguillarum. They also indicated that plasmids among strains which belonged to the traditional fish pathogenic serogroups, O1 and O2, showed more homology than did strains from most other serogroups, that were usually non-pathogenic, environmental bacteria.
Original languageEnglish
JournalEPIDEMIOLOGY AND INFECTION
Volume117
Issue number3
Pages (from-to)471-478
Number of pages8
ISSN0950-2688
DOIs
Publication statusPublished - 1996
Externally publishedYes

Keywords

  • Plasmids
  • Polymorphism, Restriction Fragment Length
  • Serotyping
  • Vibrio
  • article
  • gram negative infection
  • nonhuman
  • plasmid
  • restriction fragment length polymorphism
  • restriction mapping
  • seroepidemiology
  • serotype
  • vibrio
  • PUBLIC,
  • INFECTIOUS
  • GEL-ELECTROPHORESIS
  • VIRULENCE PLASMID
  • STRAINS
  • FISH
  • DNA
  • RESISTANCE
  • RIBOTYPES
  • BACTERIA
  • SEROVAR
  • ORDALII
  • ANALYTICAL METHOD
  • FISH PATHOGEN
  • INFECTION
  • PLASMID PROFILE
  • RESTRICTION FRAGMENT LENGTH POLYMORPHISM
  • RFLP
  • SEROTYPING
  • TYPING METHOD
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio anguillarum
  • 31500, Genetics of bacteria and viruses
  • 36002, Medical and clinical microbiology - Bacteriology
  • Genetics
  • Infection

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