Plasma membrane proteome analysis identifies a role of barley membrane steroid binding protein in root architecture response to salinity: Barley plasma membrane proteins under salinity

Katja Witzel*, Andrea Matros, Anders L. B. Moller, Eswarayya Ramireddy, Christine Finnie, Manuela Peukert, Twan Rutten, Andreas Herzog, Gotthard Kunze, Michael Melzer, Stephanie Kaspar-Schoenefeld, Thomas Schmuelling, Birte Svensson, Hans-Peter Mock

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Although the physiological consequences of plant growth under saline conditions have been well described, understanding the core mechanisms conferring plant salt adaptation has only started. We target the root plasma membrane proteomes of two barley varieties, cvs. Steptoe and Morex, with contrasting salinity tolerance. In total, 588 plasma membrane proteins were identified by mass spectrometry, of which 182 were either cultivar or salinity stress responsive. Three candidate proteins with increased abundance in the tolerant cv. Morex were involved either in sterol binding (a GTPase-activating protein for the adenosine diphosphate ribosylation factor [ZIGA2], and a membrane steroid binding protein [MSBP]) or in phospholipid synthesis (phosphoethanolamine methyltransferase [PEAMT]). Overexpression of barley MSBP conferred salinity tolerance to yeast cells, whereas the knock-out of the heterologous AtMSBP1 increased salt sensitivity in Arabidopsis. Atmsbp1 plants showed a reduced number of lateral roots under salinity, and root-tip-specific expression of barley MSBP in Atmsbp1 complemented this phenotype. In barley, an increased abundance of MSBP correlates with reduced root length and lateral root formation as well as increased levels of auxin under salinity being stronger in the tolerant cv. Morex. Hence, we concluded the involvement of MSBP in phytohormone-directed adaptation of root architecture in response to salinity.
Original languageEnglish
JournalPlant, Cell and Environment
Volume41
Issue number6
Pages (from-to)1311-1330
Number of pages20
ISSN0140-7791
DOIs
Publication statusPublished - 2018

Keywords

  • Barley
  • MSBP
  • Plasma membrane
  • Proteome analysis
  • Root morphology
  • Salinity

Fingerprint Dive into the research topics of 'Plasma membrane proteome analysis identifies a role of barley membrane steroid binding protein in root architecture response to salinity: Barley plasma membrane proteins under salinity'. Together they form a unique fingerprint.

Cite this