Plasma Exosome-Enriched Extracellular Vesicles From Lactating Mothers With Type 1 Diabetes Contain Aberrant Levels of miRNAs During the Postpartum Period

Caroline Frørup, Aashiq H. Mirza, Reza Yarani, Lotte B. Nielsen, Elisabeth R. Mathiesen, Peter Damm, Jens Svare, Christian Engelbrekt, Joachim Størling, Jesper Johannesen, Henrik B. Mortensen, Flemming Pociot, Simranjeet Kaur*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

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Abstract

Type 1 diabetes is an immune-driven disease, where the insulin-producing beta cells from the pancreatic islets of Langerhans becomes target of immune-mediated destruction. Several studies have highlighted the implication of circulating and exosomal microRNAs (miRNAs) in type 1 diabetes, underlining its biomarker value and novel therapeutic potential. Recently, we discovered that exosome-enriched extracellular vesicles carry altered levels of both known and novel miRNAs in breast milk from lactating mothers with type 1 diabetes. In this study, we aimed to characterize exosomal miRNAs in the circulation of lactating mothers with and without type 1 diabetes, hypothesizing that differences in type 1 diabetes risk in offspring from these groups are reflected in the circulating miRNA profile. We performed small RNA sequencing on exosome-enriched extracellular vesicles extracted from plasma of 52 lactating mothers around 5 weeks postpartum (26 with type 1 diabetes and 26 age-matched controls), and found a total of 2,289 miRNAs in vesicles from type 1 diabetes and control libraries. Of these, 176 were differentially expressed in plasma from mothers with type 1 diabetes (167 upregulated; 9 downregulated, using a cut-off of abs(log2FC) >1 and FDR adjusted p-value
Original languageEnglish
Article number744509
JournalFrontiers in Immunology
Volume12
Number of pages1
ISSN1664-3224
DOIs
Publication statusPublished - 2021

Keywords

  • Exosomes
  • Extracellular vesicles
  • miRNAs
  • Plasma
  • Small RNA-Seq
  • Type 1 diabetes

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