Plant Polyphenols Stimulate Adhesion to Intestinal Mucosa and Induce Proteome Changes in the Probiotic Lactobacillus acidophilus NCFM

Hasan Ufuk Celebioglu, Marta Delsoglio, Susanne Brix, Enrica Pessione, Birte Svensson*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Scope: Plant phenolics, known to exert beneficial effects on human health, were supplemented to cultures of the probiotic bacterium Lactobacillus acidophilus NCFM (NCFM) to assess their effect on its adhesive capacity and the abundancy of individual proteins.

Methods and results: The presence of resveratrol and ferulic acid during bacterial growth stimulated adhesion of NCFM to mucin and human intestinal HT-29 cells, while tannic acid improved adhesion only to HT-29 cells and caffeic acid had very modest effect overall. Some dosage dependence was found for the four phenolics supplemented at 100, 250 or 500 μg/mL to the cultures. Notably, 500 μg/mL ferulic acid only stimulated adhesion to mucin. Analyses of differential whole-cell as well as surface proteomes revealed relative abundancy changes for a total of 27 and 22 NCFM proteins, respectively. These changes include enzymes acting in metabolic pathways, such as glycolysis, nucleotide metabolism and stress response as well as being known moonlighting or surface-associated proteins.

Conclusion: The five plant phenolics found in various foods stimulate the adhesive capacity of NCFM in diverse ways and elicited relative abundancy changes of specific proteins providing molecular level insight into the mechanism of the putative beneficial effects of the polyphenols.
Original languageEnglish
Article number1700638
JournalMolecular Nutrition & Food Research
Volume62
Issue number4
ISSN1613-4125
DOIs
Publication statusPublished - 2018

Cite this

@article{434164d9737a44beb3bc3f44f0f0f6d2,
title = "Plant Polyphenols Stimulate Adhesion to Intestinal Mucosa and Induce Proteome Changes in the Probiotic Lactobacillus acidophilus NCFM",
abstract = "Scope: Plant phenolics, known to exert beneficial effects on human health, were supplemented to cultures of the probiotic bacterium Lactobacillus acidophilus NCFM (NCFM) to assess their effect on its adhesive capacity and the abundancy of individual proteins.Methods and results: The presence of resveratrol and ferulic acid during bacterial growth stimulated adhesion of NCFM to mucin and human intestinal HT-29 cells, while tannic acid improved adhesion only to HT-29 cells and caffeic acid had very modest effect overall. Some dosage dependence was found for the four phenolics supplemented at 100, 250 or 500 μg/mL to the cultures. Notably, 500 μg/mL ferulic acid only stimulated adhesion to mucin. Analyses of differential whole-cell as well as surface proteomes revealed relative abundancy changes for a total of 27 and 22 NCFM proteins, respectively. These changes include enzymes acting in metabolic pathways, such as glycolysis, nucleotide metabolism and stress response as well as being known moonlighting or surface-associated proteins.Conclusion: The five plant phenolics found in various foods stimulate the adhesive capacity of NCFM in diverse ways and elicited relative abundancy changes of specific proteins providing molecular level insight into the mechanism of the putative beneficial effects of the polyphenols.",
author = "Celebioglu, {Hasan Ufuk} and Marta Delsoglio and Susanne Brix and Enrica Pessione and Birte Svensson",
year = "2018",
doi = "10.1002/mnfr.201700638",
language = "English",
volume = "62",
journal = "Molecular Nutrition & Food Research",
issn = "1613-4125",
publisher = "Wiley-VCH",
number = "4",

}

Plant Polyphenols Stimulate Adhesion to Intestinal Mucosa and Induce Proteome Changes in the Probiotic Lactobacillus acidophilus NCFM. / Celebioglu, Hasan Ufuk; Delsoglio, Marta; Brix, Susanne; Pessione, Enrica; Svensson, Birte.

In: Molecular Nutrition & Food Research, Vol. 62, No. 4, 1700638, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Plant Polyphenols Stimulate Adhesion to Intestinal Mucosa and Induce Proteome Changes in the Probiotic Lactobacillus acidophilus NCFM

AU - Celebioglu, Hasan Ufuk

AU - Delsoglio, Marta

AU - Brix, Susanne

AU - Pessione, Enrica

AU - Svensson, Birte

PY - 2018

Y1 - 2018

N2 - Scope: Plant phenolics, known to exert beneficial effects on human health, were supplemented to cultures of the probiotic bacterium Lactobacillus acidophilus NCFM (NCFM) to assess their effect on its adhesive capacity and the abundancy of individual proteins.Methods and results: The presence of resveratrol and ferulic acid during bacterial growth stimulated adhesion of NCFM to mucin and human intestinal HT-29 cells, while tannic acid improved adhesion only to HT-29 cells and caffeic acid had very modest effect overall. Some dosage dependence was found for the four phenolics supplemented at 100, 250 or 500 μg/mL to the cultures. Notably, 500 μg/mL ferulic acid only stimulated adhesion to mucin. Analyses of differential whole-cell as well as surface proteomes revealed relative abundancy changes for a total of 27 and 22 NCFM proteins, respectively. These changes include enzymes acting in metabolic pathways, such as glycolysis, nucleotide metabolism and stress response as well as being known moonlighting or surface-associated proteins.Conclusion: The five plant phenolics found in various foods stimulate the adhesive capacity of NCFM in diverse ways and elicited relative abundancy changes of specific proteins providing molecular level insight into the mechanism of the putative beneficial effects of the polyphenols.

AB - Scope: Plant phenolics, known to exert beneficial effects on human health, were supplemented to cultures of the probiotic bacterium Lactobacillus acidophilus NCFM (NCFM) to assess their effect on its adhesive capacity and the abundancy of individual proteins.Methods and results: The presence of resveratrol and ferulic acid during bacterial growth stimulated adhesion of NCFM to mucin and human intestinal HT-29 cells, while tannic acid improved adhesion only to HT-29 cells and caffeic acid had very modest effect overall. Some dosage dependence was found for the four phenolics supplemented at 100, 250 or 500 μg/mL to the cultures. Notably, 500 μg/mL ferulic acid only stimulated adhesion to mucin. Analyses of differential whole-cell as well as surface proteomes revealed relative abundancy changes for a total of 27 and 22 NCFM proteins, respectively. These changes include enzymes acting in metabolic pathways, such as glycolysis, nucleotide metabolism and stress response as well as being known moonlighting or surface-associated proteins.Conclusion: The five plant phenolics found in various foods stimulate the adhesive capacity of NCFM in diverse ways and elicited relative abundancy changes of specific proteins providing molecular level insight into the mechanism of the putative beneficial effects of the polyphenols.

U2 - 10.1002/mnfr.201700638

DO - 10.1002/mnfr.201700638

M3 - Journal article

VL - 62

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 4

M1 - 1700638

ER -