Pig major acute-phase protein and haptoglobin serum concentrations correlate with PCV2 viremia and the clinical course of postweaning multisystemic wasting syndrome

Llorenc Grau-Roma, Peter M. H. Heegaard, Charlotte Kristiane Hjulsager, Marina Sibila, Charlotte Sonne Kristensen, A. Allepuz, M. Pineiro, Lars Erik Larsen, Joaquim Segales, Lorenzo Fraile

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The aim of the present longitudinal study was to assess the evolution of two acute phase proteins (APPs), pig-major acute phase protein (pig-MAP) and haptoglobin (HPT), in serum from pigs that developed postweaning multisystemic wasting syndrome (PMWS) in comparison to healthy and wasted non-PMWS affected pigs. In addition, evidence of infection with other pathogens and its relation with variations in APP's concentrations was also assessed. Fourteen independent batches of 100 to 154 pigs were monitored from birth to PMWS outbreak occurrence in 11 PMWS affected farms. Pigs displaying PMWS-like signs and age-matched healthy controls were euthanized during the clinical outbreak. PMWS was diagnosed according to internationally accepted creteria and pigs were classified as: i)PMWS cases, ii) wasted non-PMWS cases and iii) healthy pigs. At the moment of PMWS occurrence, pig-MAP and HPT concentration in PMWS affected pigs were higher than in healthy ones (p>0.001). No differences in APPs serum concentrations between subclinically PCV2 infected pigs and healthy non-PCV2 infected pigs (based on quantitative PCR on serum results) were detected. Results showed a significant correlation between PCV2 loads and both pig-MAP (R=0.487 to 0.602, p>0.001) and HPT (R=0.326 to 0.550, p>0.05 to 0.0001) concentrations in serum of PMWS affected pigs, indicating that the acute phase response in PMWS affected pigs occurred concomitantly to PCV2 viremia. No other pathogen, apart from PCV2, was consistently related with variatiions in APP's concentrations. A ROC analysis, made to determine the capacity of discrimination of both APPs between PMWS affected and non-affected pigs, showed higher sensitivity and specificity values using pig-MAP compared to HPT. These results suggest that pig-MAP might be a better indicator of PMWS status than HPT. Moreover, the fact that APR occurred some days before the starting of clinical signs suggests that APPs could provide valuable prognostic information for PMWS development.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume138
    Issue number1-2
    Pages (from-to)53-61
    ISSN0378-1135
    DOIs
    Publication statusPublished - 2009

    Fingerprint Dive into the research topics of 'Pig major acute-phase protein and haptoglobin serum concentrations correlate with PCV2 viremia and the clinical course of postweaning multisystemic wasting syndrome'. Together they form a unique fingerprint.

    Cite this