Picosecond thermometer in the amide I band of myoglobin

R.H. Austin, A. Xie, L. van der Meer, B. Redlich, Per-Anker Lindgård, H. Frauenfelder, D. Fu

    Research output: Contribution to journalJournal articleResearchpeer-review

    545 Downloads (Pure)


    The amide I and II bands in myoglobin show a heterogeneous temperature dependence, with bands at 6.17 and 6.43 mu m which are more intense at low temperatures. The amide I band temperature dependence is on the long wavelength edge of the band, while the short wavelength side has almost no temperature dependence. We compare concepts of anharmonic solid-state crystal physics and chemical physics for the origins of these bands. We suggest that the long wavelength side is composed of those amino acids which hydrogen bond to the hydration shell of the protein, and that temperature dependent bands can be used to determine the time it takes vibrational energy to flow into the hydration shell. We determine that vibrational energy flow to the hydration shell from the amide I takes approximately 20 ps to occur.
    Original languageEnglish
    JournalPhysical Review Letters
    Issue number12
    Pages (from-to)128101
    Number of pages4
    Publication statusPublished - 2005

    Bibliographical note

    Copyright 2005 American Physical Society


    Dive into the research topics of 'Picosecond thermometer in the amide I band of myoglobin'. Together they form a unique fingerprint.

    Cite this