Picosecond thermometer in the amide I band of myoglobin

R.H. Austin, A. Xie, L. van der Meer, B. Redlich, Per-Anker Lindgård, H. Frauenfelder, D. Fu

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    Abstract

    The amide I and II bands in myoglobin show a heterogeneous temperature dependence, with bands at 6.17 and 6.43 mu m which are more intense at low temperatures. The amide I band temperature dependence is on the long wavelength edge of the band, while the short wavelength side has almost no temperature dependence. We compare concepts of anharmonic solid-state crystal physics and chemical physics for the origins of these bands. We suggest that the long wavelength side is composed of those amino acids which hydrogen bond to the hydration shell of the protein, and that temperature dependent bands can be used to determine the time it takes vibrational energy to flow into the hydration shell. We determine that vibrational energy flow to the hydration shell from the amide I takes approximately 20 ps to occur.
    Original languageEnglish
    JournalPhysical Review Letters
    Volume94
    Issue number12
    Pages (from-to)128101
    Number of pages4
    ISSN0031-9007
    DOIs
    Publication statusPublished - 2005

    Bibliographical note

    Copyright 2005 American Physical Society

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