Pichia pastoris yeast as a vehicle for oral vaccination of larval and adult teleosts

Carmen W.E. Embregts, Felipe Reyes-Lopez, Adina C. Pall, Ansgar Stratmann, Luis Tort, Niels Lorenzen, Kirsten Engell-Sorensen, Geert F. Wiegertjes, Maria Forlenza, J. Oriol Sunyer, David Parra*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Oral vaccination is of major interest because it can be used for mass vaccination of fish of various size and age. Given that their administration is relatively easy and stress-free, oral vaccines have both economic and animal welfare benefits. Yet, mostly due to their limited efficacy, only very few oral vaccines are available to aquaculture industry. Here we present a method for oral vaccine delivery based on the yeast Pichia pastoris. We could express a model antigen, green fluorescent protein (GFP), in this yeast and subsequently show delivery of the GFP protein to the intestine of juvenile flounder or adult carp and trout. We tested this approach in several commercially-relevant fish species, from juvenile to adult stage. To test the oral delivery of antigen to larval fish, the GFP-expressing Pichia pastoris was first fed to planktonic crustacean Daphnia or rotifers that served as ‘bioencapsulation vehicles’ and afterwards, fed to flounder larvae. Again, we could show delivery of intact GFP protein to the intestine. In rainbow trout, the orally-administered GFP-expressing yeast elicited a rapid local innate immune response in the intestine and a subsequent systemic response in the spleen. Our results show that Pichia pastoris is a good vehicle for oral antigen delivery and that it can be used in non-encapsulated form for older fish or in bioencapsulated form for larval fish. We discuss the immunomodulatory properties of the yeast itself, and its potential to enhance local immune responses and act as an adjuvant.

Original languageEnglish
JournalFish and Shellfish Immunology
Volume85
Pages (from-to)52-60
ISSN1050-4648
DOIs
Publication statusPublished - 1 Feb 2019

Cite this

Embregts, C. W. E., Reyes-Lopez, F., Pall, A. C., Stratmann, A., Tort, L., Lorenzen, N., ... Parra, D. (2019). Pichia pastoris yeast as a vehicle for oral vaccination of larval and adult teleosts. Fish and Shellfish Immunology, 85, 52-60. https://doi.org/10.1016/j.fsi.2018.07.033
Embregts, Carmen W.E. ; Reyes-Lopez, Felipe ; Pall, Adina C. ; Stratmann, Ansgar ; Tort, Luis ; Lorenzen, Niels ; Engell-Sorensen, Kirsten ; Wiegertjes, Geert F. ; Forlenza, Maria ; Sunyer, J. Oriol ; Parra, David. / Pichia pastoris yeast as a vehicle for oral vaccination of larval and adult teleosts. In: Fish and Shellfish Immunology. 2019 ; Vol. 85. pp. 52-60.
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title = "Pichia pastoris yeast as a vehicle for oral vaccination of larval and adult teleosts",
abstract = "Oral vaccination is of major interest because it can be used for mass vaccination of fish of various size and age. Given that their administration is relatively easy and stress-free, oral vaccines have both economic and animal welfare benefits. Yet, mostly due to their limited efficacy, only very few oral vaccines are available to aquaculture industry. Here we present a method for oral vaccine delivery based on the yeast Pichia pastoris. We could express a model antigen, green fluorescent protein (GFP), in this yeast and subsequently show delivery of the GFP protein to the intestine of juvenile flounder or adult carp and trout. We tested this approach in several commercially-relevant fish species, from juvenile to adult stage. To test the oral delivery of antigen to larval fish, the GFP-expressing Pichia pastoris was first fed to planktonic crustacean Daphnia or rotifers that served as ‘bioencapsulation vehicles’ and afterwards, fed to flounder larvae. Again, we could show delivery of intact GFP protein to the intestine. In rainbow trout, the orally-administered GFP-expressing yeast elicited a rapid local innate immune response in the intestine and a subsequent systemic response in the spleen. Our results show that Pichia pastoris is a good vehicle for oral antigen delivery and that it can be used in non-encapsulated form for older fish or in bioencapsulated form for larval fish. We discuss the immunomodulatory properties of the yeast itself, and its potential to enhance local immune responses and act as an adjuvant.",
author = "Embregts, {Carmen W.E.} and Felipe Reyes-Lopez and Pall, {Adina C.} and Ansgar Stratmann and Luis Tort and Niels Lorenzen and Kirsten Engell-Sorensen and Wiegertjes, {Geert F.} and Maria Forlenza and Sunyer, {J. Oriol} and David Parra",
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Embregts, CWE, Reyes-Lopez, F, Pall, AC, Stratmann, A, Tort, L, Lorenzen, N, Engell-Sorensen, K, Wiegertjes, GF, Forlenza, M, Sunyer, JO & Parra, D 2019, 'Pichia pastoris yeast as a vehicle for oral vaccination of larval and adult teleosts', Fish and Shellfish Immunology, vol. 85, pp. 52-60. https://doi.org/10.1016/j.fsi.2018.07.033

Pichia pastoris yeast as a vehicle for oral vaccination of larval and adult teleosts. / Embregts, Carmen W.E.; Reyes-Lopez, Felipe; Pall, Adina C.; Stratmann, Ansgar; Tort, Luis; Lorenzen, Niels; Engell-Sorensen, Kirsten; Wiegertjes, Geert F.; Forlenza, Maria; Sunyer, J. Oriol; Parra, David.

In: Fish and Shellfish Immunology, Vol. 85, 01.02.2019, p. 52-60.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Pichia pastoris yeast as a vehicle for oral vaccination of larval and adult teleosts

AU - Embregts, Carmen W.E.

AU - Reyes-Lopez, Felipe

AU - Pall, Adina C.

AU - Stratmann, Ansgar

AU - Tort, Luis

AU - Lorenzen, Niels

AU - Engell-Sorensen, Kirsten

AU - Wiegertjes, Geert F.

AU - Forlenza, Maria

AU - Sunyer, J. Oriol

AU - Parra, David

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Oral vaccination is of major interest because it can be used for mass vaccination of fish of various size and age. Given that their administration is relatively easy and stress-free, oral vaccines have both economic and animal welfare benefits. Yet, mostly due to their limited efficacy, only very few oral vaccines are available to aquaculture industry. Here we present a method for oral vaccine delivery based on the yeast Pichia pastoris. We could express a model antigen, green fluorescent protein (GFP), in this yeast and subsequently show delivery of the GFP protein to the intestine of juvenile flounder or adult carp and trout. We tested this approach in several commercially-relevant fish species, from juvenile to adult stage. To test the oral delivery of antigen to larval fish, the GFP-expressing Pichia pastoris was first fed to planktonic crustacean Daphnia or rotifers that served as ‘bioencapsulation vehicles’ and afterwards, fed to flounder larvae. Again, we could show delivery of intact GFP protein to the intestine. In rainbow trout, the orally-administered GFP-expressing yeast elicited a rapid local innate immune response in the intestine and a subsequent systemic response in the spleen. Our results show that Pichia pastoris is a good vehicle for oral antigen delivery and that it can be used in non-encapsulated form for older fish or in bioencapsulated form for larval fish. We discuss the immunomodulatory properties of the yeast itself, and its potential to enhance local immune responses and act as an adjuvant.

AB - Oral vaccination is of major interest because it can be used for mass vaccination of fish of various size and age. Given that their administration is relatively easy and stress-free, oral vaccines have both economic and animal welfare benefits. Yet, mostly due to their limited efficacy, only very few oral vaccines are available to aquaculture industry. Here we present a method for oral vaccine delivery based on the yeast Pichia pastoris. We could express a model antigen, green fluorescent protein (GFP), in this yeast and subsequently show delivery of the GFP protein to the intestine of juvenile flounder or adult carp and trout. We tested this approach in several commercially-relevant fish species, from juvenile to adult stage. To test the oral delivery of antigen to larval fish, the GFP-expressing Pichia pastoris was first fed to planktonic crustacean Daphnia or rotifers that served as ‘bioencapsulation vehicles’ and afterwards, fed to flounder larvae. Again, we could show delivery of intact GFP protein to the intestine. In rainbow trout, the orally-administered GFP-expressing yeast elicited a rapid local innate immune response in the intestine and a subsequent systemic response in the spleen. Our results show that Pichia pastoris is a good vehicle for oral antigen delivery and that it can be used in non-encapsulated form for older fish or in bioencapsulated form for larval fish. We discuss the immunomodulatory properties of the yeast itself, and its potential to enhance local immune responses and act as an adjuvant.

U2 - 10.1016/j.fsi.2018.07.033

DO - 10.1016/j.fsi.2018.07.033

M3 - Journal article

C2 - 30016686

AN - SCOPUS:85050493589

VL - 85

SP - 52

EP - 60

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

ER -