Phytases for Improved Iron Absorption

Anne Veller Friis Nielsen, Christian Nyffenegger, Anne S. Meyer

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Abstract

Microbial phytases (EC 3.1.3.8) catalyse dephosphorylation of phytic acid, which is the primary storage compound for phosphorous in cereal kernels. The negatively charged phosphates in phytic acid chelate iron (Fe3+) and thus retards iron bioavailability in humans 1. Supplementation of microbial phytase can improve iron absorption from cereal-based diets 2. In order for phytase to catalyse iron release in vivo the phytase must be robust to low pH and proteolysis in the gastric ventricle. Our work has compared the robustness of five different microbial phytases, evaluating thermal stability, activity retention, and extent of dephosphorylation of phytic acid in a simulated low pH/pepsin gastric environment. The five phytases responded differently to the robustness parameters: The Peniophora lycii phytase (Ronozyme NP) was the most thermostable, but the least robust enzyme at low pH, whereas the two tested Aspergillus niger phytases (SukaPhy phytase and a cloned A. niger enzyme), and an Escherichia coli phytase proved to be most resistant to low pH and pepsin hydrolysis. The phytase from Citrobacter braakii (Ronozyme HiPhos) showed intermediate robustness.
Original languageEnglish
Title of host publicationAbstract Book - DTU Sustain Conference 2014
Number of pages1
Place of PublicationKgs. Lyngby
PublisherTechnical University of Denmark
Publication date2014
Publication statusPublished - 2014
EventDTU Sustain Conference 2014 - Technical University of Denmark, Lyngby, Denmark
Duration: 17 Dec 201417 Dec 2014
http://www.sustain.dtu.dk/

Conference

ConferenceDTU Sustain Conference 2014
LocationTechnical University of Denmark
CountryDenmark
CityLyngby
Period17/12/201417/12/2014
Internet address

Cite this

Nielsen, A. V. F., Nyffenegger, C., & Meyer, A. S. (2014). Phytases for Improved Iron Absorption. In Abstract Book - DTU Sustain Conference 2014 Technical University of Denmark.