Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

Jana Lišková, Timothy E.L. Douglas*, Robbe Wijnants, Sangram Keshari Samal, Ana Carina Loureiro Mendes, Ioannis S. Chronakis, Lucie Bačáková, Andre G. Skirtach

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.
Original languageEnglish
JournalMaterials Letters
Pages (from-to)186-189
Number of pages4
Publication statusPublished - 2018


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