Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

Jana Lišková, Timothy E.L. Douglas*, Robbe Wijnants, Sangram Keshari Samal, Ana Carina Loureiro Mendes, Ioannis S. Chronakis, Lucie Bačáková, Andre G. Skirtach

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.
Original languageEnglish
JournalMaterials Letters
Volume214
Pages (from-to)186-189
Number of pages4
ISSN0167-577X
DOIs
Publication statusPublished - 2018

Cite this

Lišková, Jana ; Douglas, Timothy E.L. ; Wijnants, Robbe ; Samal, Sangram Keshari ; Mendes, Ana Carina Loureiro ; Chronakis, Ioannis S. ; Bačáková, Lucie ; Skirtach, Andre G. / Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels. In: Materials Letters. 2018 ; Vol. 214. pp. 186-189.
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title = "Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels",
abstract = "Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.",
author = "Jana Liškov{\'a} and Douglas, {Timothy E.L.} and Robbe Wijnants and Samal, {Sangram Keshari} and Mendes, {Ana Carina Loureiro} and Chronakis, {Ioannis S.} and Lucie Bač{\'a}kov{\'a} and Skirtach, {Andre G.}",
year = "2018",
doi = "10.1016/j.matlet.2017.12.004",
language = "English",
volume = "214",
pages = "186--189",
journal = "Materials Letters",
issn = "0167-577X",
publisher = "Elsevier",

}

Lišková, J, Douglas, TEL, Wijnants, R, Samal, SK, Mendes, ACL, Chronakis, IS, Bačáková, L & Skirtach, AG 2018, 'Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels', Materials Letters, vol. 214, pp. 186-189. https://doi.org/10.1016/j.matlet.2017.12.004

Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels. / Lišková, Jana; Douglas, Timothy E.L.; Wijnants, Robbe; Samal, Sangram Keshari; Mendes, Ana Carina Loureiro; Chronakis, Ioannis S.; Bačáková, Lucie; Skirtach, Andre G.

In: Materials Letters, Vol. 214, 2018, p. 186-189.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

AU - Lišková, Jana

AU - Douglas, Timothy E.L.

AU - Wijnants, Robbe

AU - Samal, Sangram Keshari

AU - Mendes, Ana Carina Loureiro

AU - Chronakis, Ioannis S.

AU - Bačáková, Lucie

AU - Skirtach, Andre G.

PY - 2018

Y1 - 2018

N2 - Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.

AB - Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.

U2 - 10.1016/j.matlet.2017.12.004

DO - 10.1016/j.matlet.2017.12.004

M3 - Journal article

VL - 214

SP - 186

EP - 189

JO - Materials Letters

JF - Materials Letters

SN - 0167-577X

ER -