Physiological functions of osmolality and calcium ions on the initiation of sperm motility and swimming performance in redside dace, Clinostomus elongatus

Ian A. E. Butts, Sayyed Mohammad Hadi Alavi, Ali Mokdad, Trevor E. Pitcher

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Reproductive potential of fish stocks is critically dependent on sperm performance in an aquatic environment. The aim of this study is to test hypotheses, which govern the initiation of sperm motility and swimming performance, through physiological functions of osmolality and Ca2+ ion, in a threatened species of freshwater fish, the redside dace, Clinostomus elongatus. Spermatozoa motility was activated in either ionic or non-ionic media spanning a range of osmolalities. The role of Ca2+ channels on induction of spermatozoa motility and velocity was experimentally investigated by diluting sperm in media that contain various Ca2+ channel blockers. Results show that initiation of spermatozoa motility is a hypo-osmolality dependent mechanism. Inhibitors for L-type Ca2+ channels partially prohibited initiation of spermatozoa motility, while velocity was significantly reduced in both L-type and T-type Ca2+ channel blockers. Examination using W-7, an inhibitor for Ca2+-dependent calmodulin, showed significant decreases in spermatozoa motility and velocity. Involvement for Ca2+ in axonemal beating was confirmed by significant increases in velocity after adding Ca2+ into the activation media, while motility remained unchanged in Ca2+ supplemented activation media. Together, these findings suggest the involvement of Ca2+ in hypo-osmolality-dependent initiation of spermatozoa motility mediated by activation of Ca2+ binding protein in the axoneme of a freshwater fish sperm. Blocking Ca2+ exchange through L- or T-type Ca2+ channel influences flagellar beating force and leads to decrease in spermatozoa velocity. (C) 2013 Elsevier Inc. All rights reserved.
Original languageEnglish
JournalComparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
Volume166
Issue number1
Pages (from-to)147-157
ISSN1095-6433
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Ca2+ channel blocker
  • Osmolality
  • Ion
  • Sperm
  • Cyprinid
  • Reproduction
  • FISH reproduction
  • Animals
  • Calcium
  • Calcium Channel Blockers
  • Cyprinidae
  • Ions
  • Kinetics
  • Male
  • Osmolar Concentration
  • Rivers
  • Sperm Motility
  • Swimming
  • SY7Q814VUP Calcium
  • BIOCHEMISTRY
  • PHYSIOLOGY
  • ZOOLOGY
  • CARP CYPRINUS-CARPIO
  • FRESH-WATER TELEOSTS
  • SALMONID FISH SPERM
  • OUTER ARM DYNEIN
  • COMMON CARP
  • SEMINAL PLASMA
  • RAINBOW-TROUT
  • SPERMATOZOA
  • POTASSIUM
  • CHANNELS
  • axonemal beating
  • cell motility
  • endanger species
  • osmolality
  • physiological function
  • reproductive potential
  • swimming performance
  • Pisces Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Osteichthyes [85206] Clinostomus elongatus species redside dace common mature protected species male
  • calcium channel
  • calcium ion 14127-61-8
  • L-type calcium ion channel
  • W-7 259104-93-3 Ca2+-dependent calmodulin inhibitor
  • 00512, General biology - Conservation and resource management
  • 07002, Behavioral biology - General and comparative behavior
  • 07003, Behavioral biology - Animal behavior
  • 07502, Ecology: environmental biology - General and methods
  • 07516, Ecology: environmental biology - Wildlife management: aquatic
  • 10060, Biochemistry studies - General
  • 10069, Biochemistry studies - Minerals
  • 10508, Biophysics - Membrane phenomena
  • 16504, Reproductive system - Physiology and biochemistry
  • Conservation
  • flagella
  • sperm reproductive system
  • spermatozoa reproductive system
  • Behavior
  • Biochemistry and Molecular Biophysics
  • Reproductive System
  • Wildlife Management
  • calcium binding protein
  • calcium ion
  • calmodulin
  • article
  • axoneme
  • Clinostomus elongatus
  • controlled study
  • Cypriniformes
  • flagellum
  • freshwater fish
  • nonhuman
  • performance
  • spermatozoon motility
  • swimming
  • velocity

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