Physico-chemical and colloidal properties of protein extracted from black soldier fly (Hermetia illucens) larvae

Lucas Sales Queiroz; Marine Regnard; Flemming Jessen; Mohammad Amin Mohammadifar; Jens J. Sloth; Heidi Olander Petersen; Fatemeh Ajalloueian; Chloé Marie Charlotte Brouzes; Wael Fraihi; Heather Fallquist; Antonio Fernandes de Carvalho; Federico Casanova

doi:10.1016/j.ijbiomac.2021.07.081

Physico-chemical and colloidal properties of protein extracted from black soldier fly (Hermetia illucens) larvae

Lucas Sales Queiroz, Marine Regnard, Flemming Jessen, Mohammad Amin Mohammadifar, Jens J. Sloth, Heidi Olander Petersen, Fatemeh Ajalloueian, Chloé Marie Charlotte Brouzes, Wael Fraihi, Heather Fallquist, Antonio Fernandes de Carvalho, Federico Casanova^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

The black soldier fly larvae (BSFL), Hermetia illucens (Linnaeus), has been largely utilized for animal feed. Due to its interesting composition, BSFL has great potential to be further implemented in the human diet. Herein we compared the flour and protein extract composition based on their moisture, ash, amino acids, mineral, and protein content. To have wide knowledge on protein profile and behavior, SDS-page electrophoresis, Fourier-transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) were used to give information about protein structure and thermal stability, respectively. The flour and protein extract contained respectively 37.3% and 61.1% of protein. DSC graph reported a glass transition temperature around 30 °C, recognizable by a shift in the curve, and an endothermic peak for solid melting at around 200 °C. FTIR analysis showed the main amide bands (A, B, I, II, III) for the flour and protein extract. The foam properties of BSFL protein extract were explored under different temperatures treatment, and the best foam stability was reached at 85 °C with 15 min of treatment. The data highlight the promising techno-functional properties of BSFL protein extract, and that the nutritional composition might be suitable for further use of BSFL as food fortification system.

Original language	English
Journal	International Journal of Biological Macromolecules
Volume	186
Pages (from-to)	714-723
ISSN	0141-8130
DOIs	https://doi.org/10.1016/j.ijbiomac.2021.07.081
Publication status	Published - 1 Sept 2021

Bibliographical note

Funding Information:
The authors are grateful for the financial support of CAPES (Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior), CNPq (Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico) and FAPEMIG (Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais). The authors acknowledge the founding ERASMUS Internship for Marine Regnard. The authors acknowledge Ms. Inge Holmberg from Research Group for Bioactives - Analysis and Application (DTU, Denmark) to provide the analysis of amino acid composition.

Funding Information:
The authors are grateful for the financial support of CAPES ( Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ), CNPq ( Conselho Nacional de Desenvolvimento Científico e Tecnológico ) and FAPEMIG ( Fundação de Amparo à Pesquisa do Estado de Minas Gerais ). The authors acknowledge the founding ERASMUS Internship for Marine Regnard. The authors acknowledge Ms. Inge Holmberg from Research Group for Bioactives - Analysis and Application (DTU, Denmark) to provide the analysis of amino acid composition.

Publisher Copyright:
© 2021 The Authors

Keywords

Black soldier fly larvae
BSFL flour
Foaming properties
Protein extraction and characterization

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Queiroz, L. S., Regnard, M., Jessen, F., Mohammadifar, M. A., Sloth, J. J., Petersen, H. O., Ajalloueian, F., Brouzes, C. M. C., Fraihi, W., Fallquist, H., de Carvalho, A. F., & Casanova, F. (2021). Physico-chemical and colloidal properties of protein extracted from black soldier fly (Hermetia illucens) larvae. International Journal of Biological Macromolecules, 186, 714-723. https://doi.org/10.1016/j.ijbiomac.2021.07.081

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abstract = "The black soldier fly larvae (BSFL), Hermetia illucens (Linnaeus), has been largely utilized for animal feed. Due to its interesting composition, BSFL has great potential to be further implemented in the human diet. Herein we compared the flour and protein extract composition based on their moisture, ash, amino acids, mineral, and protein content. To have wide knowledge on protein profile and behavior, SDS-page electrophoresis, Fourier-transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) were used to give information about protein structure and thermal stability, respectively. The flour and protein extract contained respectively 37.3% and 61.1% of protein. DSC graph reported a glass transition temperature around 30 °C, recognizable by a shift in the curve, and an endothermic peak for solid melting at around 200 °C. FTIR analysis showed the main amide bands (A, B, I, II, III) for the flour and protein extract. The foam properties of BSFL protein extract were explored under different temperatures treatment, and the best foam stability was reached at 85 °C with 15 min of treatment. The data highlight the promising techno-functional properties of BSFL protein extract, and that the nutritional composition might be suitable for further use of BSFL as food fortification system.",

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author = "Queiroz, {Lucas Sales} and Marine Regnard and Flemming Jessen and Mohammadifar, {Mohammad Amin} and Sloth, {Jens J.} and Petersen, {Heidi Olander} and Fatemeh Ajalloueian and Brouzes, {Chlo{\'e} Marie Charlotte} and Wael Fraihi and Heather Fallquist and {de Carvalho}, {Antonio Fernandes} and Federico Casanova",

note = "Funding Information: The authors are grateful for the financial support of CAPES (Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior), CNPq (Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico) and FAPEMIG (Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais). The authors acknowledge the founding ERASMUS Internship for Marine Regnard. The authors acknowledge Ms. Inge Holmberg from Research Group for Bioactives - Analysis and Application (DTU, Denmark) to provide the analysis of amino acid composition. Funding Information: The authors are grateful for the financial support of CAPES ( Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior ), CNPq ( Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico ) and FAPEMIG ( Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de Minas Gerais ). The authors acknowledge the founding ERASMUS Internship for Marine Regnard. The authors acknowledge Ms. Inge Holmberg from Research Group for Bioactives - Analysis and Application (DTU, Denmark) to provide the analysis of amino acid composition. Publisher Copyright: {\textcopyright} 2021 The Authors",

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Queiroz, LS, Regnard, M, Jessen, F, Mohammadifar, MA , Sloth, JJ , Petersen, HO , Ajalloueian, F, Brouzes, CMC, Fraihi, W, Fallquist, H, de Carvalho, AF & Casanova, F 2021, 'Physico-chemical and colloidal properties of protein extracted from black soldier fly (Hermetia illucens) larvae', International Journal of Biological Macromolecules, vol. 186, pp. 714-723. https://doi.org/10.1016/j.ijbiomac.2021.07.081

TY - JOUR

T1 - Physico-chemical and colloidal properties of protein extracted from black soldier fly (Hermetia illucens) larvae

AU - Queiroz, Lucas Sales

AU - Regnard, Marine

AU - Jessen, Flemming

AU - Mohammadifar, Mohammad Amin

AU - Sloth, Jens J.

AU - Petersen, Heidi Olander

AU - Ajalloueian, Fatemeh

AU - Brouzes, Chloé Marie Charlotte

AU - Fraihi, Wael

AU - Fallquist, Heather

AU - de Carvalho, Antonio Fernandes

AU - Casanova, Federico

N1 - Funding Information: The authors are grateful for the financial support of CAPES (Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior), CNPq (Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico) and FAPEMIG (Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais). The authors acknowledge the founding ERASMUS Internship for Marine Regnard. The authors acknowledge Ms. Inge Holmberg from Research Group for Bioactives - Analysis and Application (DTU, Denmark) to provide the analysis of amino acid composition. Funding Information: The authors are grateful for the financial support of CAPES ( Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ), CNPq ( Conselho Nacional de Desenvolvimento Científico e Tecnológico ) and FAPEMIG ( Fundação de Amparo à Pesquisa do Estado de Minas Gerais ). The authors acknowledge the founding ERASMUS Internship for Marine Regnard. The authors acknowledge Ms. Inge Holmberg from Research Group for Bioactives - Analysis and Application (DTU, Denmark) to provide the analysis of amino acid composition. Publisher Copyright: © 2021 The Authors

PY - 2021/9/1

Y1 - 2021/9/1

N2 - The black soldier fly larvae (BSFL), Hermetia illucens (Linnaeus), has been largely utilized for animal feed. Due to its interesting composition, BSFL has great potential to be further implemented in the human diet. Herein we compared the flour and protein extract composition based on their moisture, ash, amino acids, mineral, and protein content. To have wide knowledge on protein profile and behavior, SDS-page electrophoresis, Fourier-transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) were used to give information about protein structure and thermal stability, respectively. The flour and protein extract contained respectively 37.3% and 61.1% of protein. DSC graph reported a glass transition temperature around 30 °C, recognizable by a shift in the curve, and an endothermic peak for solid melting at around 200 °C. FTIR analysis showed the main amide bands (A, B, I, II, III) for the flour and protein extract. The foam properties of BSFL protein extract were explored under different temperatures treatment, and the best foam stability was reached at 85 °C with 15 min of treatment. The data highlight the promising techno-functional properties of BSFL protein extract, and that the nutritional composition might be suitable for further use of BSFL as food fortification system.

AB - The black soldier fly larvae (BSFL), Hermetia illucens (Linnaeus), has been largely utilized for animal feed. Due to its interesting composition, BSFL has great potential to be further implemented in the human diet. Herein we compared the flour and protein extract composition based on their moisture, ash, amino acids, mineral, and protein content. To have wide knowledge on protein profile and behavior, SDS-page electrophoresis, Fourier-transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) were used to give information about protein structure and thermal stability, respectively. The flour and protein extract contained respectively 37.3% and 61.1% of protein. DSC graph reported a glass transition temperature around 30 °C, recognizable by a shift in the curve, and an endothermic peak for solid melting at around 200 °C. FTIR analysis showed the main amide bands (A, B, I, II, III) for the flour and protein extract. The foam properties of BSFL protein extract were explored under different temperatures treatment, and the best foam stability was reached at 85 °C with 15 min of treatment. The data highlight the promising techno-functional properties of BSFL protein extract, and that the nutritional composition might be suitable for further use of BSFL as food fortification system.

KW - Black soldier fly larvae

KW - BSFL flour

KW - Foaming properties

KW - Protein extraction and characterization

U2 - 10.1016/j.ijbiomac.2021.07.081

DO - 10.1016/j.ijbiomac.2021.07.081

M3 - Journal article

C2 - 34274399

AN - SCOPUS:85110384049

SN - 0141-8130

VL - 186

SP - 714

EP - 723

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Physico-chemical and colloidal properties of protein extracted from black soldier fly (Hermetia illucens) larvae

Abstract

Bibliographical note

Keywords

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