Phylogenetic characterisation of the G(L) sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark

Lars Erik Larsen, Torben Storgaard, Elisabeth Holm

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The study describes for the first time the phylogenetic relationship between equine arteritis virus (EAV) isolated from asymptomatic virus-shedding stallions and fatal cases of equine viral arteritis (EVA) in an European country. EAV was isolated from three dead foals and an aborted foetus during three different outbreaks of EVA. From these fatalities, the complete open reading frame 5, encoding the EAV GL protein, was amplified by reverse transcription-polymerase chain reaction and subjected to nucleotide sequence analysis. Furthermore, DNA sequences were obtained from virus isolated from semen samples of seven virus-shedding, but clinically healthy, Danish stallions. DNA sequence alignment revealed an overall divergence of 0-14 and 0-10% at the nucleotide and amino acid levels, respectively. Phylogenetic analysis including 24 previously published sequences revealed that European as well as North American "types" of EAV were present in the semen of asymptomatic carrier stallions and in fatal cases of EVA. Our results reveal that the presence of EAV-shedding stallions in Denmark represents a potential source of severe EVA.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume80
    Issue number4
    Pages (from-to)339-346
    ISSN0378-1135
    DOIs
    Publication statusPublished - 2001

    Keywords

    • phylogeny
    • equine arteritis virus
    • horse
    • open reading frame 5
    • G(L) protein
    • epidemiology

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