Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences

S. Svitashev, T. Bryngelsson, A. Vershinin, C. Pedersen, T. Säll, R. von Bothmer

    Research output: Contribution to journalJournal articleResearch

    Abstract

    A set of six cloned barley (Hordeum vulgare) repetitive DNA sequences was used for the analysis of phylogenetic relationships among 31 species (46 taxa) of the genus Hordeum, using molecular hybridization techniques. In situ hybridization experiments showed dispersed organization of the sequences over all chromosomes of H. vulgare and the wild barley species H. bulbosum, H. marinum and H. murinum. Southern blot hybridization revealed different levels of polymorphism among barley species and the RFLP data were used to generate a phylogenetic tree for the genus Hordeum. Our data are in a good agreement with the classification system which suggests the division of the genus into four major groups, containing the genomes I, X, Y, and H. However, our investigation also supports previous molecular studies of barley species where the unique position of H. bulbosum has been pointed out. In our experiments, H. bulbosum generally had hybridization patterns different from those of H. vulgare, although both carry the I genome. Based on our results we present a hypothesis concerning the possible origin and phylogeny of the polyploid barley species H. secalinum, H. depressum and the H. brachyantherum complex.
    Original languageEnglish
    JournalTheoretical and Applied Genetics
    Volume89
    Issue number7-8
    Pages (from-to)801-810
    ISSN0040-5752
    Publication statusPublished - 1994

    Fingerprint Dive into the research topics of 'Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences'. Together they form a unique fingerprint.

    Cite this