Abstract
| Original language | English |
|---|---|
| Journal | Journal of the American Chemical Society |
| Number of pages | 9 |
| ISSN | 0002-7863 |
| DOIs | |
| Publication status | Published - 2018 |
Keywords
- Photolysis
- Dynamic Nuclear Polarization
- Hyperpolarization
- Benzoylformic acid
- Dihydroxyacetone
Cite this
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Photogenerated Radical in Phenylglyoxylic Acid for in Vivo Hyperpolarized 13C MR with Photosensitive Metabolic Substrates. / Marco-Rius, Irene; Cheng, Tian; Gaunt, Adam P.; Patel, Saket; Kreis, Felix; Capozzi, Andrea; Wright, Alan J.; Brindle, Kevin M.; Ouari, Olivier; Comment, Arnaud.
In: Journal of the American Chemical Society, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Photogenerated Radical in Phenylglyoxylic Acid for in Vivo Hyperpolarized 13C MR with Photosensitive Metabolic Substrates
AU - Marco-Rius, Irene
AU - Cheng, Tian
AU - Gaunt, Adam P.
AU - Patel, Saket
AU - Kreis, Felix
AU - Capozzi, Andrea
AU - Wright, Alan J.
AU - Brindle, Kevin M.
AU - Ouari, Olivier
AU - Comment, Arnaud
PY - 2018
Y1 - 2018
N2 - Whether for 13C magnetic resonance studies in chemistry,biochemistry, or biomedicine, hyperpolarization methods based on dynamicnuclear polarization (DNP) have become ubiquitous. DNP requires asource of unpaired electrons, which are commonly added to the sampleto be hyperpolarized in the form of stable free radicals. Once polarized,the presence of these radicals is unwanted. These radicals can bereplaced by nonpersistent radicals created by the photoirradiationof pyruvic acid (PA), which are annihilated upon dissolution or thermalizationin the solid state. However, since PA is readily metabolized by mostcells, its presence may be undesirable for some metabolic studies.In addition, some 13C substrates are photosensitive andtherefore may degrade during the photogeneration of a PA radical,which requires ultraviolet (UV) light. We show here that the photoirradiationof phenylglyoxylic acid (PhGA) using visible light produces a nonpersistentradical that, in principle, can be used to hyperpolarize any molecule.We compare radical yields in samples containing PA and PhGA upon photoirradiationwith broadband and narrowband UV–visible light sources. Todemonstrate the suitability of PhGA as a radical precursor for DNP,we polarized the gluconeogenic probe 13C-dihydroxyacetone,which is UV-sensitive, using a commercial 3.35 T DNP polarizer andthen injected this into a mouse and followed its metabolism in vivo.
AB - Whether for 13C magnetic resonance studies in chemistry,biochemistry, or biomedicine, hyperpolarization methods based on dynamicnuclear polarization (DNP) have become ubiquitous. DNP requires asource of unpaired electrons, which are commonly added to the sampleto be hyperpolarized in the form of stable free radicals. Once polarized,the presence of these radicals is unwanted. These radicals can bereplaced by nonpersistent radicals created by the photoirradiationof pyruvic acid (PA), which are annihilated upon dissolution or thermalizationin the solid state. However, since PA is readily metabolized by mostcells, its presence may be undesirable for some metabolic studies.In addition, some 13C substrates are photosensitive andtherefore may degrade during the photogeneration of a PA radical,which requires ultraviolet (UV) light. We show here that the photoirradiationof phenylglyoxylic acid (PhGA) using visible light produces a nonpersistentradical that, in principle, can be used to hyperpolarize any molecule.We compare radical yields in samples containing PA and PhGA upon photoirradiationwith broadband and narrowband UV–visible light sources. Todemonstrate the suitability of PhGA as a radical precursor for DNP,we polarized the gluconeogenic probe 13C-dihydroxyacetone,which is UV-sensitive, using a commercial 3.35 T DNP polarizer andthen injected this into a mouse and followed its metabolism in vivo.
KW - Photolysis
KW - Dynamic Nuclear Polarization
KW - Hyperpolarization
KW - Benzoylformic acid
KW - Dihydroxyacetone
U2 - 10.1021/jacs.8b09326
DO - 10.1021/jacs.8b09326
M3 - Journal article
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
ER -