Photobacterium damselae subsp damselae, an emerging pathogen in Danish rainbow trout, Oncorhynchus mykiss (Walbaum), mariculture

Karl Pedersen, Helle Frank Skall, Anne Marie Lassen-Nielsen, Lotte Bjerrum Friis-Holm, Niels Jørgen Olesen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A selection of 16 field isolates of Photobacterium damselae from marine rainbow trout farms in Denmark was subjected to phenotypic and genotypic characterization and pathogenicity to fish. All isolates belonged to the subspecies damselae, being positive for haemolysis, motility and urease. There were considerable differences in haemolytic properties, some isolates presenting a broad zone of haemolysis and others only a narrow zone. Pulsed-field gel electrophoresis revealed a high diversity indicating that P. damselae subsp. damselae is an opportunistic, not clonal pathogen in Danish marine rainbow trout. Virulence of the strains to rainbow trout was highly variable with LD50 values ranging from 3.9 x 10(3) to 1.5 x 10(8) cfu at 20 degrees C. The virulence was significantly higher at 20 degrees C than at 13 degrees C. The strains with the strongest haemolytic properties were the most virulent suggesting a strong involvement of haemolysin in the pathogenesis. The pathological changes were consistent with a bacterial septicaemia and the haemorrhages were more pronounced than for most other bacterial infections.
    Original languageEnglish
    JournalJournal of Fish Diseases
    Volume32
    Issue number5
    Pages (from-to)465-472
    ISSN0140-7775
    DOIs
    Publication statusPublished - 2009

    Keywords

    • mariculture
    • damselae
    • Photobacterium damselae subsp
    • Denmark
    • vibriosis
    • rainbow trout

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