In this work, we employ frequency-domain photoacoustic microscopy to obtain photoacoustic images of labeled and unlabeled cells. The photoacoustic microscope is based on an intensity-modulated diode laser in combination with a focused piezo-composite transducer and allows imaging of labeled cells without severe photo-bleaching. We demonstrate that frequency-domain photoacoustic microscopy realized with a diode laser is capable of recording photoacoustic images of single cells with sub-μm resolution. As examples, we present images of undyed human red blood cells, stained human epithelial cells, and stained yeast cells.
|Conference||SPIE Photonics West 2018|
|Period||27/01/2018 → …|
|Series||Proceedings of SPIE, the International Society for Optical Engineering|
- Photoacoustic microscope
- Diode laser