Photoacoustic microscopy of single cells employing an intensity-modulated diode laser

Gregor Langer; Bianca Buchegger; Jaroslaw Jacak; Manoj Kumar Dasa; Thomas A. Klar; Thomas Berer

doi:10.1117/12.2289978

Photoacoustic microscopy of single cells employing an intensity-modulated diode laser

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Manoj Kumar Dasa, Thomas A. Klar, Thomas Berer

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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Abstract

In this work, we employ frequency-domain photoacoustic microscopy to obtain photoacoustic images of labeled and unlabeled cells. The photoacoustic microscope is based on an intensity-modulated diode laser in combination with a focused piezo-composite transducer and allows imaging of labeled cells without severe photo-bleaching. We demonstrate that frequency-domain photoacoustic microscopy realized with a diode laser is capable of recording photoacoustic images of single cells with sub-μm resolution. As examples, we present images of undyed human red blood cells, stained human epithelial cells, and stained yeast cells.

Original language	English
Title of host publication	Proceedings of SPIE
Number of pages	7
Volume	10494
Publisher	SPIE - International Society for Optical Engineering
Publication date	2018
Article number	104942L
DOIs	https://doi.org/10.1117/12.2289978
Publication status	Published - 2018
Event	SPIE Photonics West BiOS 2018 - Moscone Center, San Francisco, United States Duration: 27 Jan 2018 → 1 Feb 2018 https://spie.org/PWB/conferencedetails/multiphoton-microscopy

Conference

Conference	SPIE Photonics West BiOS 2018
Location	Moscone Center
Country/Territory	United States
City	San Francisco
Period	27/01/2018 → 01/02/2018
Internet address	https://spie.org/PWB/conferencedetails/multiphoton-microscopy

Series	Proceedings of SPIE, the International Society for Optical Engineering
Volume	104942
ISSN	0361-0748

Keywords

Photoacoustic microscope
Frequency-domain
Diode laser

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104942LFinal published version, 538 KB

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title = "Photoacoustic microscopy of single cells employing an intensity-modulated diode laser",

abstract = "In this work, we employ frequency-domain photoacoustic microscopy to obtain photoacoustic images of labeled and unlabeled cells. The photoacoustic microscope is based on an intensity-modulated diode laser in combination with a focused piezo-composite transducer and allows imaging of labeled cells without severe photo-bleaching. We demonstrate that frequency-domain photoacoustic microscopy realized with a diode laser is capable of recording photoacoustic images of single cells with sub-μm resolution. As examples, we present images of undyed human red blood cells, stained human epithelial cells, and stained yeast cells.",

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author = "Gregor Langer and Bianca Buchegger and Jaroslaw Jacak and Dasa, {Manoj Kumar} and Klar, {Thomas A.} and Thomas Berer",

year = "2018",

doi = "10.1117/12.2289978",

language = "English",

volume = "10494",

series = "Proceedings of SPIE, the International Society for Optical Engineering",

booktitle = "Proceedings of SPIE",

publisher = "SPIE - International Society for Optical Engineering",

note = "SPIE Photonics West BiOS 2018 ; Conference date: 27-01-2018 Through 01-02-2018",

url = "https://spie.org/PWB/conferencedetails/multiphoton-microscopy",

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Langer, G, Buchegger, B, Jacak, J, Dasa, MK, Klar, TA & Berer, T 2018, Photoacoustic microscopy of single cells employing an intensity-modulated diode laser. in Proceedings of SPIE . vol. 10494, 104942L, SPIE - International Society for Optical Engineering, Proceedings of SPIE, the International Society for Optical Engineering, vol. 104942, SPIE Photonics West BiOS 2018, San Francisco, California, United States, 27/01/2018. https://doi.org/10.1117/12.2289978

Photoacoustic microscopy of single cells employing an intensity-modulated diode laser. / Langer, Gregor; Buchegger, Bianca; Jacak, Jaroslaw et al.
Proceedings of SPIE . Vol. 10494 SPIE - International Society for Optical Engineering, 2018. 104942L (Proceedings of SPIE, the International Society for Optical Engineering, Vol. 104942).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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AU - Dasa, Manoj Kumar

AU - Klar, Thomas A.

AU - Berer, Thomas

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AB - In this work, we employ frequency-domain photoacoustic microscopy to obtain photoacoustic images of labeled and unlabeled cells. The photoacoustic microscope is based on an intensity-modulated diode laser in combination with a focused piezo-composite transducer and allows imaging of labeled cells without severe photo-bleaching. We demonstrate that frequency-domain photoacoustic microscopy realized with a diode laser is capable of recording photoacoustic images of single cells with sub-μm resolution. As examples, we present images of undyed human red blood cells, stained human epithelial cells, and stained yeast cells.

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KW - Diode laser

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DO - 10.1117/12.2289978

M3 - Article in proceedings

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T3 - Proceedings of SPIE, the International Society for Optical Engineering

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T2 - SPIE Photonics West BiOS 2018

Y2 - 27 January 2018 through 1 February 2018

ER -

Photoacoustic microscopy of single cells employing an intensity-modulated diode laser

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