Photoacoustic microscopy of single cells employing an intensity-modulated diode laser

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Manoj Kumar Dasa, Thomas A. Klar, Thomas Berer

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Abstract

In this work, we employ frequency-domain photoacoustic microscopy to obtain photoacoustic images of labeled and unlabeled cells. The photoacoustic microscope is based on an intensity-modulated diode laser in combination with a focused piezo-composite transducer and allows imaging of labeled cells without severe photo-bleaching. We demonstrate that frequency-domain photoacoustic microscopy realized with a diode laser is capable of recording photoacoustic images of single cells with sub-μm resolution. As examples, we present images of undyed human red blood cells, stained human epithelial cells, and stained yeast cells.
Original languageEnglish
Title of host publicationProceedings of SPIE
Number of pages7
Volume10494
PublisherSPIE - International Society for Optical Engineering
Publication date2018
Article number104942L
DOIs
Publication statusPublished - 2018
EventSPIE Photonics West 2018: SPIE BIOS: Design and Quality for Biomedical Technologies XI -
Duration: 27 Jan 2018 → …

Conference

ConferenceSPIE Photonics West 2018
Period27/01/2018 → …
SeriesProceedings of SPIE, the International Society for Optical Engineering
Volume104942
ISSN0361-0748

Keywords

  • Photoacoustic microscope
  • Frequency-domain
  • Diode laser

Cite this

Langer, G., Buchegger, B., Jacak, J., Dasa, M. K., Klar, T. A., & Berer, T. (2018). Photoacoustic microscopy of single cells employing an intensity-modulated diode laser. In Proceedings of SPIE (Vol. 10494). [104942L] SPIE - International Society for Optical Engineering. Proceedings of SPIE, the International Society for Optical Engineering, Vol.. 104942 https://doi.org/10.1117/12.2289978