TY - JOUR
T1 - Phosphate sensing by fluorecent reporter proteins embedded in poly-acrylamide nanoparticles
AU - Sun, Honghao
AU - Scharff-Poulsen, Anne Marie
AU - Gu, Hong
AU - Jakobsen, Iver
AU - Kossmann, Jens Matthias
AU - Frommer, W.B.
AU - Almdal, Kristoffer
PY - 2008
Y1 - 2008
N2 - Phosphate sensors were developed by embedding fluorescent reporter proteins (FLIPPi) in polyacrylamide nanoparticles; with diameters from 40 to 120 nm. The sensor activity and protein loading efficiency varied according to nanoparticle composition, that is, the total monomer content (% T) and the cross-linker content (% C). Nanoparticles with 28% T and 20% C were considered optimal as a result of relatively high loading efficiency (50.6%) as well as high protein activity (50%). The experimental results prove that the cross-linked polyacrylamide matrix could protect FLIPPi from degradation by soluble proteases to some extent. This nanoparticle embedding method provides a novel promising tool for in vivo metabolite studies. It also demonstrates a universal method for embedding different fragile bioactive elements, such as antibodies, genes, enzymes, and other functional proteins, in nanoparticles for, for example, sensing, biological catalysis, and gene delivery.
AB - Phosphate sensors were developed by embedding fluorescent reporter proteins (FLIPPi) in polyacrylamide nanoparticles; with diameters from 40 to 120 nm. The sensor activity and protein loading efficiency varied according to nanoparticle composition, that is, the total monomer content (% T) and the cross-linker content (% C). Nanoparticles with 28% T and 20% C were considered optimal as a result of relatively high loading efficiency (50.6%) as well as high protein activity (50%). The experimental results prove that the cross-linked polyacrylamide matrix could protect FLIPPi from degradation by soluble proteases to some extent. This nanoparticle embedding method provides a novel promising tool for in vivo metabolite studies. It also demonstrates a universal method for embedding different fragile bioactive elements, such as antibodies, genes, enzymes, and other functional proteins, in nanoparticles for, for example, sensing, biological catalysis, and gene delivery.
U2 - 10.1021/nn700166x
DO - 10.1021/nn700166x
M3 - Journal article
C2 - 19206543
SN - 1936-0851
VL - 2
SP - 19
EP - 24
JO - ACS Nano
JF - ACS Nano
IS - 1
ER -