Phloem unloading in Arabidopsis roots is convective and regulated by the phloem-pole pericycle

Research output: Contribution to journalJournal article – Annual report year: 2017Researchpeer-review

Documents

DOI

  • Author: Ross-Elliott, Timothy J.

    Washington State University Pullman, United States

  • Author: Jensen, Kaare Hartvig

    Biophysics and Fluids, Department of Physics, Technical University of Denmark, Fysikvej, 2800, Kgs. Lyngby, Denmark

  • Author: Haaning, Katrine S

    Technical University of Denmark, Denmark

  • Author: Wager, Brittney M.

    Washington State University Pullman, United States

  • Author: Knoblauch, Jan

    Washington State University Pullman, United States

  • Author: Howell, Alexander H.

    Washington State University Pullman, United States

  • Author: Mullendore, Daniel L.

    Washington State University Pullman, United States

  • Author: Monteith, Alexander G

    Oxford Brookes University, United Kingdom

  • Author: Paultre, Danae

    University of Edinburgh, United Kingdom

  • Author: Yan, Dawei

    University of Cambridge, United Kingdom

  • Author: Otero, Sofia

    University of Cambridge, United Kingdom

  • Author: Bourdon, Matthieu

    University of Cambridge, United Kingdom

  • Author: Sager, Ross

    University of Delaware, United States

  • Author: Lee, Jung-Youn

    University of Delaware, United States

  • Author: Helariutta, Ykä

    University of Cambridge, United Kingdom

  • Author: Knoblauch, Michael

    Washington State University Pullman, United States

  • Author: Oparka, Karl J.

    University of Edinburgh, United Kingdom

View graph of relations

In plants, a complex mixture of solutes and macromolecules is transported by the phloem. Here, we examined how solutes and macromolecules are separated when they exit the phloem during the unloading process. We used a combination of approaches (non-invasive imaging, 3D-electron microscopy, and mathematical modelling) to show that phloem unloading of solutes in Arabidopsis roots occurs through plasmodesmata by a combination of mass flow and diffusion (convective phloem unloading). During unloading, solutes and proteins are diverted into the phloem-pole pericycle, a tissue connected to the protophloem by a unique class of 'funnel plasmodesmata'. While solutes are unloaded without restriction, large proteins are released through funnel plasmodesmata in discrete pulses, a phenomenon we refer to as 'batch unloading'. Unlike solutes, these proteins remain restricted to the phloem-pole pericycle. Our data demonstrate a major role for the phloem-pole pericycle in regulating phloem unloading in roots.
Original languageEnglish
Article numbere24125
JournaleLife
Volume6
Number of pages31
ISSN2050-084X
DOIs
Publication statusPublished - 2017

Bibliographical note

This article is distributed under the terms of the Creative Commons Attribution License

CitationsWeb of Science® Times Cited: No match on DOI

Download statistics

No data available

ID: 130762260