Phenotypic characterization of polysaccharidases produced by four Prevotella type strains

H. Matsui, K. Ogata, K. Tajima, M. Nakamura, T. Nagamine, Rustam Aminov, Y. Benno

Research output: Contribution to journalJournal articleResearchpeer-review

187 Downloads (Pure)

Abstract

Four ruminal Prevotella type strains, P. ruminicola JCM8958(T), P. bryantii B(1)4(T), P. albensis M384(T), and P. brevis ATCC19188(T), were characterized for polysaccharide-degrading activities with the reducing sugar release assay and zymogram analyses. Carboxymethylcellulase, xylanase, and polygalacturonate (PG)-degrading enzyme activities were determined in cultures grown on oat spelt xylan, xylose, arabinose, cellobiose, and glucose as sole growth substrates. P. ruminicola and P. albensis showed carboxymethylcellulase induction patterns. When xylan was supplied as a sole growth substrate, xylanase activities produced by P. bryantii and P. albensis were at least 18- and 11-fold higher, respectively, than during growth on other carbohydrates, suggesting that the regulation of the xylanases was highly specific to xylan. All strains constitutively produced PC-degrading enzymes. The corresponding activity of P. bryantii was more than 40-fold higher than in other strains. Zymogram analyses routinely detected the presence of high-molecular-weight (100-170 kDa) polysaccharide-degrading enzymes in ruminal Prevotella. Characteristics of the polysaccharide-degrading activities showed diversity of ruminal Prevotella species.
Original languageEnglish
JournalCurrent Microbiology
Volume41
Issue number1
Pages (from-to)45-49
Number of pages5
ISSN0343-8651
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Animals
  • Cellulase
  • Glycoside Hydrolases
  • Phenotype
  • Polygalacturonase
  • Polysaccharides
  • Prevotella
  • Ruminants
  • Xylan Endo-1,3-beta-Xylosidase
  • Xylans
  • Xylosidases
  • EC 3.2.1.- Glycoside Hydrolases
  • EC 3.2.1.- Xylosidases
  • EC 3.2.1.15 Polygalacturonase
  • EC 3.2.1.32 Xylan Endo-1,3-beta-Xylosidase
  • EC 3.2.1.4 Cellulase
  • EC 3.2.1.4 carboxymethylcellulase
  • arabinose
  • carboxymethylcellulose
  • cellobiose
  • cellulase
  • glucose
  • polygalacturonase
  • polygalacturonic acid
  • polysaccharide
  • xylan
  • xylan endo 1,3 beta xylosidase
  • xylose
  • article
  • bacterial growth
  • carbohydrate metabolism
  • controlled study
  • enzyme activity
  • enzyme induction
  • molecular weight
  • nonhuman
  • phenotype
  • prevotella albensis
  • prevotella brevis
  • Prevotella bryantii
  • Prevotella ruminicola
  • priority journal
  • ruminant stomach
  • MICROBIOLOGY
  • BACTEROIDES-RUMINICOLA
  • FIBER DIGESTION
  • RUMEN
  • BACTERIA
  • DIVERSITY
  • CLONING
  • B(1)4
  • 1,4-BETA-D-ENDOGLUCANASE
  • IDENTIFICATION
  • EXPRESSION
  • bacterial diversity
  • bacterial growth characteristics
  • phenotypes
  • rumen microbiology
  • Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Prevotella spp.
  • enzymes
  • polysaccharidases 9075-53-0 analysis, functions, phenotypic characterization, production
  • polysaccharides
  • proteins
  • 10060, Biochemistry studies - General
  • 10808, Enzymes - Physiological studies
  • 13002, Metabolism - General metabolism and metabolic pathways
  • 14001, Digestive system - General and methods
  • 30000, Bacteriology, general and systematic
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • 32000, Microbiological apparatus, methods and media
  • 36002, Medical and clinical microbiology - Bacteriology
  • Biochemistry and Molecular Biophysics
  • Enzymology
  • Molecular Genetics

Fingerprint Dive into the research topics of 'Phenotypic characterization of polysaccharidases produced by four Prevotella type strains'. Together they form a unique fingerprint.

Cite this