Phenotypic and genotypic characterization of Vibrio vulnificus: Proposal for the substitution of the subspecific taxon biotype for serovar

Elena G. Biosca, C. Amaro, Jens Laurits Larsen, Karl Pedersen

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Abstract

The classification of Vibrio vulnificus strains into two biotypes has been maintained on the basis of phenotypic properties and eel virulence. Biotype 2 is virulent for eels, negative for the indole reaction, and serologically homogeneous (serogroup E), whereas strains of biotype 1 are avirulent, indole positive, and serologically heterogeneous. In the present study, we phenotypically and genotypically characterized 21 V. vulnificus isolates, recovered mainly from northern Europe, by comparing them with reference strains of both biotypes to look for new isolates of biotype 2. The results of this work revealed that the majority of isolates virulent for eels presented phenotypic traits previously considered characteristics of biotype 2 and specific ribotypes with HindIII. However, among the new isolates we found (i) a serogroup E strain virulent for eels but indole positive and (ii) one isolate not belonging to serogroup E but pathogenic for eels. Since no biochemical test or specific serogroup can with certainty be associated with eel virulence, we propose to classify V. vulnificus strains into serovars instead of biotypes. Thus, we suggest serovar E as the denomination of those strains previously classified as biotype 2, Finally, the occurrence of serogroup E in eels cultured in Norway and Sweden, as well as from human infections and shrimp, has been demonstrated.
Original languageEnglish
JournalAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume63
Issue number4
Pages (from-to)1460-1466
Number of pages7
ISSN0099-2240
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • Bacterial Typing Techniques
  • Humans
  • Vibrio
  • indole
  • adolescent
  • article
  • bacterial virulence
  • bacterium identification
  • biochemistry
  • eel
  • nonhuman
  • ribotyping
  • serotyping
  • taxonomy
  • vibrio
  • Anguilliformes
  • Bacteria (microorganisms)
  • Decapoda (Crustacea)
  • Vibrio vulnificus
  • MICROBIOLOGY
  • BIOTECHNOLOGY
  • EUROPEAN EEL
  • IDENTIFICATION
  • VIRULENCE
  • PLASMID
  • ANGUILLARUM
  • CAPSULE
  • Europe Palearctic region
  • Norway Europe Palearctic region
  • Sweden Europe Palearctic region
  • BIOTYPE CLASSIFICATION
  • GENETICS
  • GENOTYPE
  • PHENOTYPE
  • RIBOTYPES
  • SEROGROUPS
  • SYSTEMATICS
  • Crustacea Arthropoda Invertebrata Animalia (Animals, Arthropods, Crustaceans, Invertebrates) - Malacostraca [75112] shrimp
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio vulnificus
  • Pisces Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Osteichthyes [85206] eel
  • Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] human
  • 00504, General biology - Taxonomy, nomenclature and terminology
  • 30000, Bacteriology, general and systematic
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • 36002, Medical and clinical microbiology - Bacteriology
  • Genetics
  • Infection
  • Physiology
  • Systematics and Taxonomy

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