Phase microscopy of technical and biological samples through random phase modulation with a difuser

Percival Almoro; Giancarlo Pedrini; Phanindra Narayan Gundu; Wolfgang Osten; Steen Grüner Hanson

doi:10.1364/OL.35.001028

Phase microscopy of technical and biological samples through random phase modulation with a difuser

Percival Almoro, Giancarlo Pedrini, Phanindra Narayan Gundu, Wolfgang Osten, Steen Grüner Hanson

Universität Stuttgart

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Abstract

A technique for phase microscopy using a phase diffuser and a reconstruction algorithm is proposed. A magnified specimen wavefront is projected on the diffuser plane that modulates the wavefront into a speckle field. The speckle patterns at axially displaced planes are sampled and used in an iterative phase retrieval algorithm based on a wave-propagation equation. The technique offers a whole-field and high-resolution wavefront reconstruction of unstained microstructures. Phase maps of photoresist targets and human cheek cells are obtained to demonstrate the effectiveness of our method. (C) 2010 Optical Society of America

Original language	English
Journal	Optics Letters
Volume	35
Issue number	7
Pages (from-to)	1028-1030
ISSN	0146-9592
DOIs	https://doi.org/10.1364/OL.35.001028
Publication status	Published - 2010

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http://www.opticsinfobase.org/abstract.cfm?uri=ol-35-7-1028

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title = "Phase microscopy of technical and biological samples through random phase modulation with a difuser",

abstract = "A technique for phase microscopy using a phase diffuser and a reconstruction algorithm is proposed. A magnified specimen wavefront is projected on the diffuser plane that modulates the wavefront into a speckle field. The speckle patterns at axially displaced planes are sampled and used in an iterative phase retrieval algorithm based on a wave-propagation equation. The technique offers a whole-field and high-resolution wavefront reconstruction of unstained microstructures. Phase maps of photoresist targets and human cheek cells are obtained to demonstrate the effectiveness of our method. (C) 2010 Optical Society of America",

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AU - Almoro, Percival

AU - Pedrini, Giancarlo

AU - Gundu, Phanindra Narayan

AU - Osten, Wolfgang

AU - Hanson, Steen Grüner

PY - 2010

Y1 - 2010

N2 - A technique for phase microscopy using a phase diffuser and a reconstruction algorithm is proposed. A magnified specimen wavefront is projected on the diffuser plane that modulates the wavefront into a speckle field. The speckle patterns at axially displaced planes are sampled and used in an iterative phase retrieval algorithm based on a wave-propagation equation. The technique offers a whole-field and high-resolution wavefront reconstruction of unstained microstructures. Phase maps of photoresist targets and human cheek cells are obtained to demonstrate the effectiveness of our method. (C) 2010 Optical Society of America

AB - A technique for phase microscopy using a phase diffuser and a reconstruction algorithm is proposed. A magnified specimen wavefront is projected on the diffuser plane that modulates the wavefront into a speckle field. The speckle patterns at axially displaced planes are sampled and used in an iterative phase retrieval algorithm based on a wave-propagation equation. The technique offers a whole-field and high-resolution wavefront reconstruction of unstained microstructures. Phase maps of photoresist targets and human cheek cells are obtained to demonstrate the effectiveness of our method. (C) 2010 Optical Society of America

U2 - 10.1364/OL.35.001028

DO - 10.1364/OL.35.001028

M3 - Journal article

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VL - 35

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JO - Optics Letters

JF - Optics Letters

IS - 7

ER -

Phase microscopy of technical and biological samples through random phase modulation with a difuser

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